Standard Curve (Sample)
S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Kit | SAM/SAH elisa kit
S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit
Synonyms
S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH); N/A; S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit; SAM/SAH elisa kit
Assay Type
Competitive
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Upon receipt, store the SAM Conjugate, SAH Conjugate, SAM Standard and SAH Standard at -80 degree C. Store the rest of the kit at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SAM/SAH elisa kit
Background: S-adenosylmethionine (SAM) is a methyl donor involved in the transfer of a methyl group to DNA, proteins, phospholipids, RNA, and neurotransmitters. Reactions that break down and regenerate SAM have been named the SAM cycle (Figure 1). SAM-dependent methylases use SAM as a substrate to yield s-adenosylhomocysteine (SAH), which is further broken down to homocysteine and adenosine by s-adenosylhomocysteine hydrolase. The homocysteine can be regenerated to methionine and finally SAM by methionine synthases. Donation of the SAM methyl group converts SAM into SAH, the latter being a potent inhibitor of methylation. For this reason, the SAM/SAH ratio has been used as an index of methylation potential in a cell. SAH in plasma has been found to be a more sensitive indicator for vascular disease risk than homocysteine. In addition, distinct classes of riboswitches that recognize SAH but not SAH and regulate gene expression have been discovered. SAM and SAH Combo ELISA Kit is a competitive enzyme immunoassay developed for the detection and quantitation of SAM and SAH in plasma, serum, lysates, or other biological fluid samples. The kit has a detection sensitivity limit of 0.625 nM for free SAM and 0.2 uM for free SAH. Each kit provides sufficient reagents to perform up to 96 total assays for SAM or SAH, including standard curves and unknown samples.
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Product Notes
The SAM/SAH (Catalog #AAA44684) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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