Human Stromal cell Derived factor 1Alpha ELISA Kit | SDF1Alpha elisa kit
Human Stromal cell Derived factor 1Alpha ELISA Kit
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Synonyms
Stromal cell Derived factor 1Alpha; N/A; Human Stromal cell Derived factor 1Alpha ELISA Kit; SDF1Alpha elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for SDF1Alpha elisa kit
Intended Uses: This SDF-1alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SDF-1alpha. This ELISA kit for research use only!
Principle of the Assay||SDF-1alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDF-1alpha antibody and an SDF-1alpha-HRP conjugate. The assay sample and buffer are incubated together with SDF-1alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF-1alpha concentration since SDF-1alpha from samples and SDF-1alpha-HRP conjugate compete for the anti-SDF-1alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF-1alpha from the sample, fewer sites are left to bind SDF-1alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1alpha concentration in each sample is interpolated from this standard curve.
Principle of the Assay||SDF-1alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDF-1alpha antibody and an SDF-1alpha-HRP conjugate. The assay sample and buffer are incubated together with SDF-1alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF-1alpha concentration since SDF-1alpha from samples and SDF-1alpha-HRP conjugate compete for the anti-SDF-1alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF-1alpha from the sample, fewer sites are left to bind SDF-1alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1alpha concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SDF1Alpha elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,004 Da
NCBI Official Full Name
stromal cell-derived factor 1 isoform alpha
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12
NCBI Official Synonym Symbols
IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
NCBI Protein Information
stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Gene Name
CXCL12
UniProt Synonym Gene Names
SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
UniProt Entry Name
SDF1_HUMAN
