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139 results for "Signaling Proteins" - showing 100-139

IP (Immunoprecipitation) (MAP2K3 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg HeLa Whole Cell Lysate 2 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MAP2K3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :39 kDa)

MEK3/MKK3, Polyclonal Antibody (Cat# AAA27720)

Full Name
Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry) (Rabbit Anti-NONO Antibody Catalog Number: AAA23484 Paraffin Embedded Tissue: Human cardiac cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X)

NONO, Polyclonal Antibody (Cat# AAA23484)

Full Name
NONO antibody - C-terminal region
Gene Names
NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Reactivity
Tested Species Reactivity: Human (Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat)
Applications
IF, IHC, WB, IP
Purity
Protein A purified
Pricing
IP (Immunoprecipitation) (HDAC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-HDAC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HDAC2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)

HDAC2, Polyclonal Antibody (Cat# AAA27718)

Full Name
Anti-HDAC2 Antibody, Rabbit Polyclonal
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

Phospho-Histone H3-S10/T11, Polyclonal Antibody (Cat# AAA28391)

Full Name
Phospho-Histone H3-S10/T11 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

HSP90, Monoclonal Antibody (Cat# AAA27660)

Full Name
HSP90 Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Dog, Fish, Shark, Hamster
Applications
WB, IHC, ICC, IF, IP, EIA, AM
Purity
Protein G Purified
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Calretinin/Calbindin 2, Monoclonal Antibody (Cat# AAA23938)

Full Name
Calretinin/Calbindin 2 (Mesothelioma Marker)
Gene Names
CALB2; CR; CAL2; CAB29
Reactivity
Human
Applications
WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Smad1/9, Polyclonal Antibody (Cat# AAA31453)

Full Name
Phospho-Smad1/9 (Ser463/Ser465) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)

MUC1 / EMA /CD227, Monoclonal Antibody (Cat# AAA13841)

Full Name
MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of GALE using anti-GALE antibody (AAA19135).GALE was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALE Antibody (AAA19135) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GALE, Polyclonal Antibody (Cat# AAA19135)

Full Name
Anti-GALE Picoband antibody
Gene Names
GALE; SDR1E1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
DB (Dot Blot) (Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17803)

Full Name
Amyloid Fibrils (OC) Antibody: ATTO 488
Reactivity
Human. Potentially mouse and rat based on species homology.
Applications
IP, ICC, IF, IHC, EIA, WB, DB
Purity
Protein A Purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of ADA using anti-ADA antibody (AAA19140).ADA was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ADA/Adenosine Deaminase, Polyclonal Antibody (Cat# AAA19140)

Full Name
Anti-ADA/Adenosine Deaminase Picoband antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2786). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Calretinin/Calbindin 2, Monoclonal Antibody (Cat# AAA23939)

Full Name
Calretinin/Calbindin 2 (Mesothelioma Marker)
Gene Names
CALB2; CR; CAL2; CAB29
Reactivity
Human
Applications
WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
IHC (Immunohistochemistry) (Figure 7. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CD105, Polyclonal Antibody (Cat# AAA19165)

Full Name
Anti-CD105 Picoband Antibody
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

AMD1/Adometdc, Polyclonal Antibody (Cat# AAA19177)

Full Name
Anti-AMD1/Adometdc Antibody
Gene Names
AMD1; AMD; SAMDC; ADOMETDC
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry) (Figure 8. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED18, Polyclonal Antibody (Cat# AAA19179)

Full Name
Anti-MED18 Picoband Antibody
Gene Names
MED18; SRB5; p28b
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED9 using anti-MED9 antibody (AAA19180).MED9 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED9 Antibody (AAA19180) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED9, Polyclonal Antibody (Cat# AAA19180)

Full Name
Anti-MED9 Picoband Antibody
Gene Names
MED9; MED25
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (AAA19166).Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Thrombopoietin Antibody (AAA19166) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Thrombopoietin, Polyclonal Antibody (Cat# AAA19166)

Full Name
Anti-Thrombopoietin Picoband Antibody
Gene Names
Thpo; Ml; Tpo; Mgdf; Mpllg
Reactivity
Mouse, Rat
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry) (Figure 7. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED4, Polyclonal Antibody (Cat# AAA19178)

Full Name
Anti-MED4 Picoband Antibody
Gene Names
MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot) (WB Suggested Anti-GSR Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)

GSR, Polyclonal Antibody (Cat# AAA23553)

Full Name
GSR antibody - N-terminal region
Gene Names
GSR; GR; HEL-75; HEL-S-122m
Reactivity
Tested Reactivity: Human, Mouse, Zebrafish
Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot) (WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)

SHH, Polyclonal Antibody (Cat# AAA23516)

Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)

S100B, Polyclonal Antibody (Cat# AAA28759)

Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
WB, IHC-P-Leica, FC/FACS, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED15 using anti-MED15 antibody (AAA19169).MED15 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (AAA19169) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)

Full Name
Anti-MED15/Pcqap Picoband Antibody
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-RGS6 antibody (AAA19308).Overlay histogram showing MCF-7 cells stained with AAA19308 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS6 Antibody (AAA19308, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RGS6, Polyclonal Antibody (Cat# AAA19308)

Full Name
Anti-RGS6 Antibody
Gene Names
RGS6; GAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SOCS1, Polyclonal Antibody (Cat# AAA28752)

Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

LAT, Polyclonal Antibody (Cat# AAA31413)

Full Name
Phospho-LAT (Tyr161) Antibody
Gene Names
LAT; LAT1; pp36
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Zebrafish (83%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (80%), Dog (82%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IRF7, Polyclonal Antibody (Cat# AAA31458)

Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot) (Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)

Ran, Polyclonal Antibody (Cat# AAA19131)

Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IP (Immunoprecipitation) (HSPA1A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 70 kDaObserved band size: 70 kDa)

HSP70, Monoclonal Antibody (Cat# AAA27747)

Full Name
Recombinant Anti-HSP70 Antibody, Rabbit Monoclonal
Gene Names
HSPA1B; HSP72; HSPA1; HSX70; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1B
Reactivity
Human
Applications
WB, EIA, IHC-P, FC/FACS/FCM, ICC, IF, IP
Purity
Protein A
Pricing
Application Data (Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)

CD229, Monoclonal Antibody (Cat# AAA11951)

Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
FC/FACS, IP
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

AR, Polyclonal Antibody (Cat# AAA31420)

Full Name
Phospho-AR (Tyr535) Antibody
Gene Names
AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Smad2, Polyclonal Antibody (Cat# AAA31433)

Full Name
Phospho-Smad2 (Ser465+Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad2, Polyclonal Antibody (Cat# AAA31390)

Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

RhoA, Polyclonal Antibody (Cat# AAA31383)

Full Name
Phospho-RhoA (Ser188) Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Histone H2A.X, Polyclonal Antibody (Cat# AAA31320)

Full Name
Acetyl-Histone H2A.X (Lys9) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Rac1/cdc42, Polyclonal Antibody (Cat# AAA31342)

Full Name
Rac1/cdc42 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry) (At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

KAT3B/p300, Polyclonal Antibody (Cat# AAA31393)

Full Name
Phospho-KAT3B/p300 (Ser1834) Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

CD227/MUC1, Polyclonal Antibody (Cat# AAA31426)

Full Name
Phospho-CD227/MUC1 (Ser1227) Antibody
Gene Names
MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot) (FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

SRC, Monoclonal Antibody (Cat# AAA28639)

Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing

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