Search results for " Monoclonal Antibodies"

2955 results for " Monoclonal Antibodies" - showing 2950-2955

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Application Data (Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)

Application Data (Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.)

Application Data (Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm)

Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm)

CD68, Monoclonal Antibody (Cat# AAA12105)

Full Name

RAT ANTI MOUSE CD68:FITC

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

FC/FACS

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Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

Application Data (Staining of mouse bone marrow with Rat anti Mouse CD11b)

Application Data (Immunoperoxidase staining o mouse lymph node cryosection with Rat anti Mouse CD11b antibody, clone 5C6 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Published customer image: Activation of adult primary microglial cells in wild-type and IL-1 KO mice. (A) Primary microglial cells were obtained from young adult wild-type mice. The cells stain with the microglial marker CD11b, but not with the neuronal and astroglial markers, NeuN and GFAP, respectively. A few cells are stained with the oligodendroglial cell marker, MBP. NC (inset) is the primary antibody-free negative control. The microglial cells (n = 3 each group) were stimulated for 24 hours in the presence of the vehicle alone, or supplemented with IFN? or IL-4 in the presence or absence of IL-1beta. Total NO (NOx; B), TNFa (C), arginase specific activity (Arg-1 spe. act.; D) and IGF-1 (E) were determined from the media or cell suspensions. (B) NOx levels increase upon exposure of the cells to IL-1beta and in a synergistic manner upon co-treatment of cells with IL-1beta and IFN?, but not when the cotreatment is with IL-4. (C) TNFa levels increase upon exposure of the cells to IFN?, and further upon co-treatment with IL-1beta. Surprisingly, the co-treatment of the cells with IL-4 and IL-1beta induced the highest TNFa level among the experimental treatments used. (D) Arg1-specific activity increased significantly upon exposure to IL-4 and further increased when IL-4 and IL-1beta were employed together. (E) IGF-1 levels decreased with exposure of the cells to IFN? and increased in response to IL-4. The response was partially inhibited by cotreatment of the cells with IL-1beta. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the vehicle-treated group in each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; IGF-1, insulin-like growth factor.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65. doi: 10.1186/1742-2094-9-65.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse CD11b antibody, clone 5C6 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. high power)

Application Data (Published customer image BM-derived GFP+ single cells and vessel-associated cells express CD11b. Fluorescence microscopy for GFP combined with immunofluorescence detection of (A) CD11b, (B) vWF and (C) CD31, 24 hours after pMCAO. (A) Fluorescence detection of GFP and CD11b showed that most GFP+ cells co-expressed CD11b (yellow cells, indicated by arrows), and intermingled with CD11b+ host cells. Note also that a few GFP+ cells did not co-express CD11b (arrow head). Insert shows high magnification of GFP+ cells, some of which co-express CD11b, aggregated around a vessel. (B, C) Fluorescence detection of GFP and the endothelial cell markers vWF (B) and CD31 (C). Inserts show higher magnification of sections of the same vessels. Although there are indications that single vWF+ cells co-express GFP (arrows in B), this could not be reproduced using staining for CD31, and the majority of vWF+ and CD31+ cells showed no co-expression of GFP. Instead, GFP remained confined to round and elongated cells located in the juxtavascular space (insert in C). CD11b+ cells were visualized using Alexa Fluor 568-conjugated goat anti-rat IgG, vWF+ and CD31+ cells using Alexa Fluor 546-conjugated goat anti-rabbit IgG and Alexa Fluor 594-conjugated goat anti-rat IgG, respectively. Scale bars: 20 um (A-C)..From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

CD11b, Monoclonal Antibody (Cat# AAA12183)

Full Name

RAT ANTI MOUSE CD11b:FITC

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

FC/FACS

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Application Data (Staining of mouse bone marrow with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:FITC)

CD11b, Monoclonal Antibody (Cat# AAA12182)

Full Name

RAT ANTI MOUSE CD11b:FITC

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

FC/FACS

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Application Data (Staining of mouse bone marrow with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:FITC)

CD11b, Monoclonal Antibody (Cat# AAA12186)

Full Name

RAT ANTI MOUSE CD11b:RPE

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

FC/FACS

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WB (Western Blot) (FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

WB (Western Blot) (The anti-Src Mab is used in Western blot to detect Src in Jurkat cell lysate.)

IF (Immunofluorescence) (Fluorescent image of A549 cell stained with SRC Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).SRC immunoreactivity is localized to Cytoplasm significantly.)

WB (Western Blot) (Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody. AAA28639 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Anti-SRC Antibody at 1:500 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)

WB (Western Blot) (Anti-SRC Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :60 kDa Blocking/Dilution buffer:5% NFDM/TBST.)

SRC, Monoclonal Antibody (Cat# AAA28639)

Full Name

SRC Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, mouse

Applications

WB, EIA, IF

Purity

This antibody is purified through a protein G column, followed by dialysis against PBS.

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2955 results for " Monoclonal Antibodies" - showing 2950-2955

CD68, Monoclonal Antibody (Cat# AAA12105)

CD11b, Monoclonal Antibody (Cat# AAA12183)

CD11b, Monoclonal Antibody (Cat# AAA12182)

CD11b, Monoclonal Antibody (Cat# AAA12186)

SRC, Monoclonal Antibody (Cat# AAA28639)

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Clonality

Type

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2955 results for " Monoclonal Antibodies" - showing 2950-2955

CD68, Monoclonal Antibody (Cat# AAA12105)

CD11b, Monoclonal Antibody (Cat# AAA12183)

CD11b, Monoclonal Antibody (Cat# AAA12182)

CD11b, Monoclonal Antibody (Cat# AAA12186)

SRC, Monoclonal Antibody (Cat# AAA28639)

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