Human sE-Selectin ELISA Kit | SELE elisa kit

Human sE-Selectin PicoKine ELISA Kit

Cat. Num. AAA45278

• Gene Names: SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
• Reactivity: Human
• Synonyms: sE-Selectin; N/A; Human sE-Selectin PicoKine ELISA Kit; E-selectin; selectin E; CD62 antigen-like family member E; CD62E; CD62E antigen; E selectin; ELAM; ELAM-1; ELAM1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; sele; SELE elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human sE-Selectin
• Sequence Length: 610
• Samples: Cell culture supernates, serum and plasma (heparin, citrate)
• Assay Type: Sandwich
• Detection Range: 125pg/ml-8000pg/ml
• Sensitivity: < 4pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human sE-Selectin PicoKine ELISA Kit standard curve)

Related Product Information for SELE elisa kit

Principle of the assay: human sE-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sE-Selectin has been precoated onto 96-well plates. Standards(NSO, W22-P556) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sE-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sE-Selectin amount of sample captured in plate.
Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increasedexpression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homingof T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurallyand functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4 The standard product used in this kit is recombinant human sE-Selectin,excluding intercellular sE-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 126180
• NCBI GeneID: 6401
• UniProt Accession #: P16581; P16111; A2RRD6
• Molecular Weight: 66,655 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: SELE
• NCBI Official Synonym Symbols: ELAM; ESEL; CD62E; ELAM1; LECAM2
• NCBI Protein Information: E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• UniProt Gene Name: SELE
• UniProt Synonym Gene Names: ELAM1; ELAM-1; LECAM2
• UniProt Entry Name: LYAM2_HUMAN