Standard Curve (Sample)
Mouse selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit | SELP elisa kit
Mouse P-Selectin ELISA Kit
Gene Names
Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
Reactivity
Mouse
Synonyms
selectin P (granule membrane protein 140kDa, antigen CD62); N/A; Mouse P-Selectin ELISA Kit; Mouse P-Selectin ELISA kit; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane; selectin P (granule membrane protein 140kDa; antigen CD62); SELP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SELP. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
Sequence Length
768
Samples
Serum, plasma, tissue homogenates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SELP elisa kit
Introduction: P-selectin is a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In unactivated endothelial cells, it is stored in granules Weibel-Palade bodies, and alpha-granules in unactivated platelets. P-selectin plays an essential role in the initial recruitment of leukocytes (white blood cells) to the site of injury during inflammation. Thrombin is one trigger which can stimulate endothelial-cell release of P-selectin and recent studies suggest an additional Ca2+-independent pathway involved in release of P-selectin. Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different than those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses. P-selectin attaches to the actin cytoskeleton through anchor proteins that are still poorly characterized.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-selectin. Standards or samples are then 3 added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for P-selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-selectin. Standards or samples are then 3 added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for P-selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,099 Da
NCBI Official Full Name
P-selectin
NCBI Official Synonym Full Names
selectin, platelet
NCBI Official Symbol
Selp
NCBI Official Synonym Symbols
Grmp; CD62P; LECAM3; PADGEM; GMP-140
NCBI Protein Information
P-selectin; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
UniProt Protein Name
P-selectin
UniProt Gene Name
Selp
UniProt Synonym Gene Names
Grmp; GMP-140; LECAM3; PADGEM
UniProt Entry Name
LYAM3_MOUSE
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Product Notes
The Mouse SELP selp (Catalog #AAA79689) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79689 ELISA Kit recognizes Mouse SELP. It is sometimes possible for the material contained within the vial of "selectin P (granule membrane protein 140kDa, antigen CD62), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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