SDS-PAGE
Semaphorin 3F (SEMA3F) Recombinant Protein | SEMA3F recombinant protein
Recombinant Semaphorin 3F (SEMA3F)
Gene Names
SEMA3F; SEMA4; SEMAK; SEMA-IV
Applications
Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Semaphorin 3F (SEMA3F); N/A; Recombinant Semaphorin 3F (SEMA3F); SEMA3F recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
V RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFL
V RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFL
Sequence Length
785
Applicable Applications for SEMA3F recombinant protein
IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Val350~Leu486
Predicted Molecular Mass
16.6kDa
Accurate Molecular Mass
16kDa as determined by SDS-PAGE reducing conditions.
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Predicted Molecular Mass
16.6kDa
Tags
N-terminal His-Tag
Homology
Mouse 96%, rat 96%
Tissue Specificity
Breast, kidney, brain, lung, liver.
Subcellular Location
Secreted.
Traits
Freeze-dried powder
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
semaphorin-3F
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
NCBI Official Symbol
SEMA3F
NCBI Official Synonym Symbols
SEMA4; SEMAK; SEMA-IV
NCBI Protein Information
semaphorin-3F; sema IV; sema III/F; semaphorin IV; semaphorin III/F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F
UniProt Protein Name
Semaphorin-3F
UniProt Gene Name
SEMA3F
UniProt Synonym Gene Names
Sema IV
UniProt Entry Name
SEM3F_HUMAN
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Product Notes
The SEMA3F sema3f (Catalog #AAA134526) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Semaphorin 3F (SEMA3F) can be used in a range of immunoassay formats including, but not limited to, IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the SEMA3F sema3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. V RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV LFL. It is sometimes possible for the material contained within the vial of "Semaphorin 3F (SEMA3F), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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