SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualised by coomassie blue stain.)
Vaspin Recombinant Protein | SERPINA12 recombinant protein
Vaspin, 21- 414aa, Human, His tag, E Coli
Gene Names
SERPINA12; OL-64
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Vaspin; N/A; Vaspin, 21- 414aa, Human, His tag, E Coli; Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12; SERPINA12; serpin peptidase inhibitor clade A member 12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Serpin A12 precursor; OL64 OL 64; ENSG00000165953; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody; SERPINA12 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid in 20mM Tris-HCI buffer (pH 8.0) containing 0.2mM PMSF, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK
Sequence Length
21-414aa
Applicable Applications for SERPINA12 recombinant protein
SDS-PAGE
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 2-8°C for up to a week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualised by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualised by coomassie blue stain.)
Related Product Information for SERPINA12 recombinant protein
Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for SERPINA12 recombinant protein
References
Hida K., et al. (2005) PNAS. 102 (30):10610-5.; Kloting N., et al. (2006) Biochem Biophys Res Commun. 339 (1):430-6;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47 kDa (415 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
serpin A12
NCBI Official Synonym Full Names
serpin family A member 12
NCBI Official Symbol
SERPINA12
NCBI Official Synonym Symbols
OL-64
NCBI Protein Information
serpin A12
UniProt Protein Name
Serpin A12
UniProt Gene Name
SERPINA12
UniProt Synonym Gene Names
Vaspin
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Product Notes
The SERPINA12 serpina12 (Catalog #AAA48464) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Vaspin can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SERPINA12 serpina12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK. It is sometimes possible for the material contained within the vial of "Vaspin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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