Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 1 ELISA Kit | SERPINE1 elisa kit

Human plasminogen activator inhibitor 1, PAI-1 ELISA Kit

Cat. Num. AAA112251

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Human
• Synonyms: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 1; N/A; Human plasminogen activator inhibitor 1, PAI-1 ELISA Kit; Human plasminogen activator inhibitor 1; PAI-1 ELISA Kit; PAI; PAI-1; PAI1; PLANH1; plasminogen activator inhibitor-1; serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1; tPAI-1; serpin peptidase inhibitor; SERPINE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PAI-1. No significant cross-reactivity or interference between human PAI-1 and analogues was observed.
• Sequence Length: 402
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml-200 ng/ml.
• Sensitivity: The minimum detectable dose of human PAI-1 is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SERPINE1 elisa kit

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAI-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAI-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAI-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAI-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• UniProt Accession #: P05121; B7Z4S0; F8WD53
• Molecular Weight: 45,060 Da
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN