Chicken Serotonin Transporter ELISA Kit | SERT elisa kit

Chicken Serotonin Transporter ELISA Kit

Cat. Num. AAA89908

• Gene Names: SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
• Reactivity: Chicken
• Synonyms: Serotonin Transporter; N/A; Chicken Serotonin Transporter ELISA Kit; SERT elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of SERT. No significant cross-reactivity or interference between SERT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SERT and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Intended Uses: This SERT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SERT. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for SERT elisa kit

Principle of the Assay: SERT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SERT antibody and an SERT-HRP conjugate. The assay sample and buffer are incubated together with SERT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERT concentration since SERT from samples and SERT-HRP conjugate compete for the anti-SERT antibody binding site. Since the number of sites is limited, as more sites are occupied by SERT from the sample, fewer sites are left to bind SERT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERT concentration in each sample is interpolated from this standard curve.

Intended Uses: This SERT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SERT. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for SERT elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 58700442
• NCBI GeneID: 6532
• UniProt Accession #: P31645; Q5EE02; Q9NYN7; AAB26687
• Molecular Weight: 70,325 Da
• NCBI Official Full Name: serotonin transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• NCBI Official Symbol: SLC6A4
• NCBI Official Synonym Symbols: HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
• NCBI Protein Information: sodium-dependent serotonin transporter; 5HT transporter; 5-hydroxytryptamine transporter; solute carrier family 6 member 4; Na+/Cl- dependent serotonin transporter
• UniProt Protein Name: Sodium-dependent serotonin transporter
• UniProt Gene Name: SLC6A4
• UniProt Synonym Gene Names: HTT; SERT; 5HTT
• UniProt Entry Name: SC6A4_HUMAN