Standard Curve (Sample)
Goat Serotonin Transporter ELISA Kit | SERT elisa kit
Goat Serotonin Transporter ELISA Kit
Gene Names
SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
Reactivity
Goat
Synonyms
Serotonin Transporter; N/A; Goat Serotonin Transporter ELISA Kit; SERT elisa kit
Reactivity
Goat
Samples
Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SERT elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This SERT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat SERT. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY SERT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SERT antibody and an SERT-HRP conjugate. The assay sample and buffer are incubated together with SERT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERT concentration since SERT from samples and SERT-HRP conjugate compete for the anti-SERT antibody binding site. Since the number of sites is limited, as more sites are occupied by SERT from the sample, fewer sites are left to bind SERT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERT concentration in each sample is interpolated from this standard curve.
INTENDED USE This SERT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat SERT. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY SERT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SERT antibody and an SERT-HRP conjugate. The assay sample and buffer are incubated together with SERT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERT concentration since SERT from samples and SERT-HRP conjugate compete for the anti-SERT antibody binding site. Since the number of sites is limited, as more sites are occupied by SERT from the sample, fewer sites are left to bind SERT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SERT elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,325 Da
NCBI Official Full Name
serotonin transporter
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
NCBI Official Symbol
SLC6A4
NCBI Official Synonym Symbols
HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
NCBI Protein Information
sodium-dependent serotonin transporter; 5HT transporter; 5-hydroxytryptamine transporter; solute carrier family 6 member 4; Na+/Cl- dependent serotonin transporter
UniProt Protein Name
Sodium-dependent serotonin transporter
UniProt Gene Name
SLC6A4
UniProt Synonym Gene Names
HTT; SERT; 5HTT
UniProt Entry Name
SC6A4_HUMAN
Similar Products
Product Notes
The Goat SERT slc6a4 (Catalog #AAA89477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA89477 ELISA Kit recognizes Goat SERT. It is sometimes possible for the material contained within the vial of "Serotonin Transporter, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.