SDS-PAGE
Serum Amyloid A1 Recombinant Protein | A1 recombinant protein
Recombinant Human Serum Amyloid A1
Applications
Western Blot, ELISA
Purity
>99% under reducing conditions(SDS-PAGE)
Synonyms
Serum Amyloid A1; N/A; Recombinant Human Serum Amyloid A1; Serum amyloid A1; A1 recombinant protein
Host
E Coli
Specificity
Purity and molecular integrity confirmed by SDS-PAGE analysis.
Purity/Purification
>99% under reducing conditions(SDS-PAGE)
Form/Format
Lyophilized at 1mg/mL in PBS, pH 7.4.
Concentration
0.1 mg/vials (1.096 mg/mL quantified by BCA) (varies by lot)
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY
Applicable Applications for A1 recombinant protein
WB (Western Blot), ELISA
Species
Human
Tag
N-terminal 6xHis
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1mg/mL and let the lyophilized pellet dissolve completely.
Quality Control Test
BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein.
Preparation and Storage
The product is stored at -80°C. Please Aliquot the antigen, and store at -80 degree C. Avoid repeated freezing /thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for A1 recombinant protein
Expressed in E Coli with total 146 AA. Mw: 16.6 KDa (calculated). N-terminal 6xHis-tag, EK and TEV cleavage site, 42 extra AA (highlighted).
SAA is synthesized in the liver and secreted to the blood. When in the blood, SAA proteins form complexes with high density lipoproteins (HDL). It has been suggested that SAA is involved in the recycling of cholesterol from damaged tissues. Similar to C-reactive protein, SAA is a major acute phase protein in human being, it can be used in test, predicting outcomes and assessing the efficacy of treatment in patients with inflammation. SAA is a sensitive biomarker of acute renal allograft rejection and it can be used to monitor SAA in kidney transplant patients for the early detection of acute rejection episodes. In patients with myocardial infarction, SAA concentration is elevated to extremely high values and correlates with postinfarction complications and the mortality rate.
SAA is synthesized in the liver and secreted to the blood. When in the blood, SAA proteins form complexes with high density lipoproteins (HDL). It has been suggested that SAA is involved in the recycling of cholesterol from damaged tissues. Similar to C-reactive protein, SAA is a major acute phase protein in human being, it can be used in test, predicting outcomes and assessing the efficacy of treatment in patients with inflammation. SAA is a sensitive biomarker of acute renal allograft rejection and it can be used to monitor SAA in kidney transplant patients for the early detection of acute rejection episodes. In patients with myocardial infarction, SAA concentration is elevated to extremely high values and correlates with postinfarction complications and the mortality rate.
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Product Notes
The A1 (Catalog #AAA60566) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Serum Amyloid A1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSENLY FQRSFFSFLG EAFDGARDMW RAYSDMREAN YIGSDKYFHA RGNYDAAKRG PGGAWAAEVI SDARENIQRF FGHGAEDSLA DQAANEWGRS GKDPNHFRPA GLPEKY. It is sometimes possible for the material contained within the vial of "Serum Amyloid A1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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