Standard Curve (Sample)
Porcine Steroidogenic Factor 1 ELISA Kit | SF1 elisa kit
Porcine Steroidogenic Factor 1 ELISA Kit
Gene Names
NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
Reactivity
Porcine
Synonyms
Steroidogenic Factor 1; N/A; Porcine Steroidogenic Factor 1 ELISA Kit; SF1 elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SF1 elisa kit
Intended Uses: This SF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SF1. This ELISA kit for research use only!
Principle of the Assay: SF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SF1 antibody and an SF1-HRP conjugate. The assay sample and buffer are incubated together with SF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SF1 concentration since SF1 from samples and SF1-HRP conjugate compete for the anti-SF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SF1 from the sample, fewer sites are left to bind SF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SF1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SF1 antibody and an SF1-HRP conjugate. The assay sample and buffer are incubated together with SF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SF1 concentration since SF1 from samples and SF1-HRP conjugate compete for the anti-SF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SF1 from the sample, fewer sites are left to bind SF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SF1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SF1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,636 Da
NCBI Official Full Name
steroidogenic factor 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 5, group A, member 1
NCBI Official Symbol
NR5A1
NCBI Official Synonym Symbols
ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
NCBI Protein Information
steroidogenic factor 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor
UniProt Protein Name
Steroidogenic factor 1
UniProt Gene Name
NR5A1
UniProt Synonym Gene Names
AD4BP; FTZF1; SF1; SF-1; STF-1
UniProt Entry Name
STF1_HUMAN
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Product Notes
The Porcine SF1 nr5a1 (Catalog #AAA97030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA97030 ELISA Kit recognizes Porcine SF1. It is sometimes possible for the material contained within the vial of "Steroidogenic Factor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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