Application Data
(The purity of PDE1C was determined to be >90% by densitometry. Approx MW 99kDa)
PDE1C active protein
PDE1C, Active
Gene Names
PDE1C; Hcam3; hCam-3; cam-PDE 1C
Purity
>90%
Synonyms
PDE1C; N/A; PDE1C, Active; PDE1C active protein
Host
Sf9 cells
Specificity
Full length recombinant protein expressed in Sf9 cells
Purity/Purification
>90%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Specific Activity
445 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Shipping: Dry Ice
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of PDE1C was determined to be >90% by densitometry. Approx MW 99kDa)
Application Data
(The purity of PDE1C was determined to be >90% by densitometry. Approx MW 99kDa)
Application Data
(The specific activity of PDE1C was determined to be 445 nmol/min/mg as per activity assay protocol)
Application Data
(The specific activity of PDE1C was determined to be 445 nmol/min/mg as per activity assay protocol)
Related Product Information for PDE1C active protein
Description: Full-length recombinant human PDE1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE1C is a member of the phosphodiesterases (PDE) family which catalyzes the hydrolysis of cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C binds both cAMP and cGMP with high affinity and hydrolyzes both substrates with similar rates of catalysis (1). PDE1C is a calmodulin-dependent PDE and is stimulated by calcium-calmodulin complex. PDE1C is expressed at high levels in human cardiac myocytes with an intracellular distribution distinct from that of other phosphodiesterases (2). PDE1C levels decrease in all conditions that inhibited cell proliferation and PDE1C is a useful marker in studying the dynamics of proliferation and migration of various cells.
Scientific Background: PDE1C is a member of the phosphodiesterases (PDE) family which catalyzes the hydrolysis of cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C binds both cAMP and cGMP with high affinity and hydrolyzes both substrates with similar rates of catalysis (1). PDE1C is a calmodulin-dependent PDE and is stimulated by calcium-calmodulin complex. PDE1C is expressed at high levels in human cardiac myocytes with an intracellular distribution distinct from that of other phosphodiesterases (2). PDE1C levels decrease in all conditions that inhibited cell proliferation and PDE1C is a useful marker in studying the dynamics of proliferation and migration of various cells.
Product Categories/Family for PDE1C active protein
References
1. Loughney, K. et al: Isolation and characterization of cDNAs corresponding to two human calcium, calmodulin-regulated, 3-prime, 5-prime-cyclic nucleotide phosphodiesterases. J. Biol. Chem. 271: 796-806, 1996. 2. Vandeput, F. et al; Cyclic nucleotide phosphodiesterase PDE1C1 in human cardiac myocytes. J. Biol. Chem. 282: 32749-42757, 2007.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~99 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 1C (PDE1C), transcript variant 4, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 1C
NCBI Official Symbol
PDE1C
NCBI Official Synonym Symbols
Hcam3; hCam-3; cam-PDE 1C
NCBI Protein Information
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
UniProt Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
UniProt Gene Name
PDE1C
UniProt Synonym Gene Names
Cam-PDE 1C
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Product Notes
The PDE1C pde1c (Catalog #AAA73095) is an Active Protein produced from Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDE1C, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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