Application Data
(The purity was determined to be > 95% by densitometry.)
PDE4A active protein
PDE4A, Active
Gene Names
PDE4A; PDE4; DPDE2; PDE46
Purity
>95%
Synonyms
PDE4A; N/A; PDE4A, Active; PDE4A active protein
Host
Sf9 cells
Purity/Purification
>95%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ml (varies by lot)
Specific Activity
1082 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Shipping: Dry Ice
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity was determined to be > 95% by densitometry.)
Application Data
(The purity was determined to be > 95% by densitometry.)
Application Data
(The specific activity of PDE4A was determined to be 1082 nmol/min/mg as per activity assay protocol.)
Application Data
(The specific activity of PDE4A was determined to be 1082 nmol/min/mg as per activity assay protocol.)
Related Product Information for PDE4A active protein
Description: Recombinant human PDE4A (332-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzyme's sensitivity to Mg (2+), leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression (1). PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder (2).
Scientific Background: PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzyme's sensitivity to Mg (2+), leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression (1). PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder (2).
Product Categories/Family for PDE4A active protein
References
1. Goldhoff P, et al: Targeted inhibition of cyclic AMP phosphodiesterase-4 promotes brain tumor regression. Clin Cancer Res. 2008 Dec 1;14 (23):7717-25. 2. Fatemi S H, et al: Phosphodiesterase-4A expression is reduced in cerebella of patients with bipolar disorder. Psychiatr Genet. 2008 Dec;18 (6):282-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~110 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 4A (PDE4A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 4A
NCBI Official Symbol
PDE4A
NCBI Official Synonym Symbols
PDE4; DPDE2; PDE46
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
UniProt Gene Name
PDE4A
UniProt Synonym Gene Names
DPDE2
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Product Notes
The PDE4A pde4a (Catalog #AAA73103) is an Active Protein produced from Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDE4A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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