SDS-PAGE
(Purity: SDS-PAGE image of PAD Cocktail Proteins. The five PAD isoform proteins from left to right are: PAD1, PAD4, PAD3, PAD6 and PAD2. The far-right lane contains PAD Cocktail.)
PAD Cocktail Active Protein | PAD active protein
PAD Cocktail, Active
Synonyms
PAD Cocktail; N/A; PAD Cocktail, Active; PAD active protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Tag
GST
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: SDS-PAGE image of PAD Cocktail Proteins. The five PAD isoform proteins from left to right are: PAD1, PAD4, PAD3, PAD6 and PAD2. The far-right lane contains PAD Cocktail.)
SDS-PAGE
(Purity: SDS-PAGE image of PAD Cocktail Proteins. The five PAD isoform proteins from left to right are: PAD1, PAD4, PAD3, PAD6 and PAD2. The far-right lane contains PAD Cocktail.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PAD active protein
The five full length recombined PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.
The PAD family of enzymes is responsible for the catalysis of arginine-to-citrulline conversion in target proteins. There are 5 mammalian PAD isoforms (PAD1-4,6) that share ~50% sequence homology. Each PAD enzyme has its own typical subcellular localization, tissue distribution and substrate specificity, although no substrate protein has been found so far for PAD6.
The PAD family of enzymes is responsible for the catalysis of arginine-to-citrulline conversion in target proteins. There are 5 mammalian PAD isoforms (PAD1-4,6) that share ~50% sequence homology. Each PAD enzyme has its own typical subcellular localization, tissue distribution and substrate specificity, although no substrate protein has been found so far for PAD6.
Product Categories/Family for PAD active protein
References
1. Tilvawala, R et. Al: Peptidyl arginine deiminases: detection and finctional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11, 2019.
2. Clancy K.W. et al., Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol., 30, 1-6, 2016.
2. Clancy K.W. et al., Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol., 30, 1-6, 2016.
NCBI and Uniprot Product Information
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Product Notes
The PAD (Catalog #AAA73186) is an Active Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PAD Cocktail, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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