Purity Info
PDE7A active protein
PDE7A, Active recombinant protein
Gene Names
PDE7A; HCP1; PDE7
Purity
>90%
Synonyms
PDE7A; N/A; PDE7A, Active recombinant protein; CCK-4; CCK4; PTK7 Protein; PDE7A active protein
Host
Sf9 insect cells
Purity/Purification
>90%
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Specific Activity
160 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Shipping: Dry Ice
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity Info
Purity Info
Specific Activity
Specific Activity
Related Product Information for PDE7A active protein
Recombinant human PDE7A (104-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE7A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE7A is a high-affinity cAMP-specific PDE and is expressed in T cell lines, peripheral blood T lymphocytes, epithelial cell lines, airway and vascular smooth muscle cells, lung fibroblasts, and eosinophils. PDE7 plays a critical role in the regulation of human T cell function and selective PDE7 inhibitors are being examined to treat immunological and inflammatory disorders (1). PDE7A plays an important role in the regulation of osteoblastic differentiation (2). PDE7A depletion by RNAi upregulates expression of several osteogenic genes and increases mineralization.
Scientific Background: PDE7A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE7A is a high-affinity cAMP-specific PDE and is expressed in T cell lines, peripheral blood T lymphocytes, epithelial cell lines, airway and vascular smooth muscle cells, lung fibroblasts, and eosinophils. PDE7 plays a critical role in the regulation of human T cell function and selective PDE7 inhibitors are being examined to treat immunological and inflammatory disorders (1). PDE7A plays an important role in the regulation of osteoblastic differentiation (2). PDE7A depletion by RNAi upregulates expression of several osteogenic genes and increases mineralization.
Product Categories/Family for PDE7A active protein
References
1.Nakata A, et al: Potential role of phosphodiesterase 7 in human T cell function: comparative effects of two phosphodiesterase inhibitors. Clin Exp Immunol. 2002 Jun;128(3):460-6.
2.Pekkinen M, et al: Effects of phosphodiesterase 7 inhibition by RNA interference on the gene expression and differentiation of human mesenchymal stem cell-derived osteoblasts. Bone. 2008 Jul;43(1):84-91.
2.Pekkinen M, et al: Effects of phosphodiesterase 7 inhibition by RNA interference on the gene expression and differentiation of human mesenchymal stem cell-derived osteoblasts. Bone. 2008 Jul;43(1):84-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~64 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 7A (PDE7A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 7A
NCBI Official Symbol
PDE7A
NCBI Official Synonym Symbols
HCP1; PDE7
NCBI Protein Information
high affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A; TM22; phosphodiesterase isozyme 7
UniProt Protein Name
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
UniProt Gene Name
PDE7A
UniProt Entry Name
PDE7A_HUMAN
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Product Notes
The PDE7A pde7a (Catalog #AAA72929) is an Active Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDE7A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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