Mitogen Activated Kinase Kinase 1 Active Protein | MEK1 active protein
Recombinant Human Mitogen Activated Kinase Kinase 1
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Mitogen Activated Kinase Kinase 1; N/A; Recombinant Human Mitogen Activated Kinase Kinase 1; MEK1 Human; Mitogen Activated Kinase Kinase 1 Human Recombinant; MEK1; MKK1; MAPKK1; MAP2K1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; PRKMK1; MEK1 active protein
Host
Sf9 Insect Cells
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
MEK1 is supplied at a concentration of 0.09mg/ml in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.
Sterile Filtered clear solution.
Sterile Filtered clear solution.
Sequence Length
393
Source
Sf9 Insect Cells.
Biological Activity
~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30 degree C in a reaction containing 100 uM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
Preparation and Storage
Store at 4°C if entire vial will be used within 1-2 weeks.
Store frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Store frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Related Product Information for MEK1 active protein
Description: MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques.
Introduction: MAP2K1 is a member of the dual specificity protein kinase family, which plays a role as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, are recognized as extracellular signal-regulated kinases (ERKs), that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As a key player of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. MAP2K1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a thr-glu-tyr sequence located in map kinases. MEK1 activates erk1 and erk2 map kinases.
Introduction: MAP2K1 is a member of the dual specificity protein kinase family, which plays a role as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, are recognized as extracellular signal-regulated kinases (ERKs), that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As a key player of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. MAP2K1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a thr-glu-tyr sequence located in map kinases. MEK1 activates erk1 and erk2 map kinases.
Product Categories/Family for MEK1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 1
NCBI Official Symbol
MAP2K1
NCBI Official Synonym Symbols
CFC3; MEK1; MKK1; MAPKK1; PRKMK1
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
UniProt Gene Name
MAP2K1
UniProt Synonym Gene Names
MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
UniProt Entry Name
MP2K1_HUMAN
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Product Notes
The MEK1 map2k1 (Catalog #AAA38618) is an Active Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mitogen Activated Kinase Kinase 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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