Application Data
CDC7/DBF4 active protein
CDC7/DBF4, Active
Gene Names
CDC7; Hsk1; CDC7L1; HsCDC7; huCDC7
Applications
Western Blot
Purity
>85%
Synonyms
CDC7/DBF4; N/A; CDC7/DBF4, Active; CDC7/DBF4 active protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCI,pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1 mM PMSF, 25% Glycerol.
Concentration
0.1 ug/uL (varies by lot)
Sequence Positions
Full Length
Sequence Length
3222
Applicable Applications for CDC7/DBF4 active protein
WB (Western Blot), Kinase Assay
Specific activity
8.5 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships in dry ice.
Stability:1 year at -70 from date of shipment.
Shipping: Ships in dry ice.
Stability:1 year at -70 from date of shipment.
Application Data
Application Data
Application Data
Application Data
Related Product Information for CDC7/DBF4 active protein
Description: Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags.
Scientific Background: CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines (1). CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase (2).
Scientific Background: CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines (1). CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase (2).
Product Categories/Family for CDC7/DBF4 active protein
References
1. Hess, G. F. et.al: A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998. 2. Sheu, Y.-J. et.al: The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4. Nature 463: 113-117, 2010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens cell division cycle 7 (CDC7), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cell division cycle 7
NCBI Official Symbol
CDC7
NCBI Official Synonym Symbols
Hsk1; CDC7L1; HsCDC7; huCDC7
NCBI Protein Information
cell division cycle 7-related protein kinase
UniProt Protein Name
Cell division cycle 7-related protein kinase
UniProt Gene Name
CDC7
UniProt Synonym Gene Names
CDC7L1; CDC7-related kinase; HsCdc7; huCdc7
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Product Notes
The CDC7/DBF4 cdc7 (Catalog #AAA72963) is an Active Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's CDC7/DBF4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the CDC7/DBF4 cdc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC7/DBF4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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