Monkey Soluble Fms-like Tyrosine 1 ELISA Kit | sFLT1 elisa kit

Monkey Soluble Fms-like Tyrosine 1 ELISA Kit

Cat. Num. AAA16870

• Gene Names: flt1; flt1b; sFlt1; VEGFR1
• Reactivity: Monkey
• Synonyms: Soluble Fms-like Tyrosine 1; N/A; Monkey Soluble Fms-like Tyrosine 1 ELISA Kit; sFLT1 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of sFLT1. No significant cross-reactivity or interference between sFLT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLT1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Intended Uses: This sFLt-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey sFLt-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for sFLT1 elisa kit

Principle of the Assay: sFLT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sFLT1 antibody and an sFLT1-HRP conjugate. The assay sample and buffer are incubated together with sFLT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLT1 concentration since sFLT1 from samples and sFLT1-HRP conjugate compete for the anti-sFLT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLT1 from the sample, fewer sites are left to bind sFLT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLT1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This sFLT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey sFLT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 324499394
• NCBI GeneID: 544667
• Molecular Weight: 53,549 Da
• NCBI Official Full Name: soluble fms-like tyrosine kinase 1
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• NCBI Official Symbol: flt1
• NCBI Official Synonym Symbols: flt1b; sFlt1; VEGFR1
• NCBI Protein Information: vascular endothelial growth factor receptor 1; flt1a; receptor tyrosine kinase Flt1b; soluble fms-like tyrosine kinase 1
• UniProt Protein Name: Flt1 protein
• UniProt Gene Name: flt1
• UniProt Synonym Gene Names: sFlt1; sflt1
• UniProt Entry Name: A4QNT7_DANRE