Sheep Abeta1-42 ELISA Kit | Abeta1-42 elisa kit
Sheep Abeta1-42 (Amyloid Beta Peptide 1-42) ELISA Kit
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Sheep
Synonyms
Abeta1-42; N/A; Sheep Abeta1-42 (Amyloid Beta Peptide 1-42) ELISA Kit; Abeta1-42 elisa kit
Reactivity
Sheep
Specificity
This kit recognizes Sheep Abeta1-42 in samples. No significant cross-reactivity or interference between Sheep Abeta1-42 and analogues was observed.
Sequence Length
677
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
7.813-500pg/mL
Sensitivity
4.688pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Sheep Abeta1-42 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Sheep Abeta1-42 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for Abeta1-42 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep Abeta1-42 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sheep Abeta1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheep Abeta1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheep Abeta1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheep Abeta1-42. You can calculate the concentration of Sheep Abeta1-42 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sheep Abeta1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheep Abeta1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheep Abeta1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheep Abeta1-42. You can calculate the concentration of Sheep Abeta1-42 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,521 Da
NCBI Official Full Name
amyloid beta A4 protein isoform j
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein
NCBI Official Symbol
APP
NCBI Official Synonym Symbols
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
NCBI Protein Information
amyloid beta A4 protein; alzheimer disease amyloid protein; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); cerebral vascular amyloid peptide; peptidase nexin-II; preA4; protease nexin-II
UniProt Protein Name
Amyloid beta A4 protein
UniProt Gene Name
APP
UniProt Synonym Gene Names
A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
UniProt Entry Name
A4_HUMAN
