SIGLEC8 recombinant protein

Recombinant Human SIGLEC8 Protein (ECD, His Tag), HPLC-verified

Cat. Num. AAA259205

• Gene Names: SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
• Purity: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Synonyms: SIGLEC8; N/A; Recombinant Human SIGLEC8 Protein (ECD, His Tag), HPLC-verified; SAF2 Protein, Human; SIGLEC-8 Protein, Human; SIGLEC8L Protein, Human; SIGLEC8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile 20mM MES, 150mM Nacl, 10% Glycerol, 10% Trehalose, pH 6.0.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: Human
• Predicted N Terminal: Met 17
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Human SIGLEC8 (NP_055257)(Met1-Ala363) was expressed with a polyhistidine tag at the C-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

Application Data

Related Product Information for SIGLEC8 recombinant protein

Siglec-8, also known as SAF2, is a single-pass transmembrane CD33 subfamily receptor selectively expressed on human eosinophils and mast cells. Capable of exhibiting cell-type dependent inhibitory activity, siglec-8 has a N-terminal V-set Ig domain containing a sialoglycan binding site that forms strong interactions with the carboxylate group in sialic acid for ligation. In its cytoplasmic domain, siglec-8 has an immunoreceptor tyrosine-based inhibitory motif (ITIM) thought to be critical for downstream signaling and function. Engagement of Siglec-8 on cytokine-primed eosinophils promotes cell death through generation of reactive oxygen species. In contrast, siglec-8 engagement on mast cells inhibits cellular activation and mediator release. Siglec-8 agonist antibodies are currently under investigation for treatment of allergic and inflammatory diseases involving mast cells and eosinophils

References

1. Carroll DJ, et al. Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating beta2-integrin-dependent function in human eosinophils. J Allergy Clin Immunol. 2018. doi: 10.1016/j.jaci.2017.08.013.
2. Cao Y, et al. Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells. Front Immunol. 2023. doi: 10.3389/fimmu.2023.1283370.
3. Wang Y, et al. Sequence variety in the CC' loop of Siglec-8/9/3 determines the recognitions to sulfated oligosaccharides. Comput Struct Biotechnol J. 2023. doi: 10.1016/j.csbj.2023.08.014.
4. Metz M, et al. Mast cell silencing: A novel therapeutic approach for urticaria and other mast cell-mediated diseases. Allergy. 2023. doi: 10.1111/all.15850.

NCBI and Uniprot Product Information

• NCBI GI #: 94538343
• NCBI GeneID: 27181
• NCBI Accession #: NP_055257
• NCBI GenBank Nucleotide #: NM_014442
• UniProt Accession #: Q9NYZ4; Q7Z728
• Molecular Weight: 46,434 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 8
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 8
• NCBI Official Symbol: SIGLEC8
• NCBI Official Synonym Symbols: SAF2; SIGLEC-8; SIGLEC8L
• NCBI Protein Information: sialic acid-binding Ig-like lectin 8; CDw329; SAF-2; sialoadhesin family member 2
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 8
• UniProt Gene Name: SIGLEC8
• UniProt Synonym Gene Names: SAF2; Siglec-8; SAF-2
• UniProt Entry Name: SIGL8_HUMAN