Porcine Soluble interleukin 6 receptor ELISA Kit | sIL-6R elisa kit

Porcine Soluble interleukin 6 receptor ELISA Kit

Cat. Num. AAA91038

• Gene Names: IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• Reactivity: Porcine
• Synonyms: Soluble interleukin 6 receptor; N/A; Porcine Soluble interleukin 6 receptor ELISA Kit; sIL-6R elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of sIL6R. No significant cross-reactivity or interference between sIL6R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sIL6R and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for sIL-6R elisa kit

Principle of the Assay: sIL6R ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sIL6R antibody and an sIL6R-HRP conjugate. The assay sample and buffer are incubated together with sIL6R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sIL6R concentration since sIL6R from samples and sIL6R-HRP conjugate compete for the anti-sIL6R antibody binding site. Since the number of sites is limited, as more sites are occupied by sIL6R from the sample, fewer sites are left to bind sIL6R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIL6R concentration in each sample is interpolated from this standard curve.

Intended Uses: This sIL6R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine sIL6R. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for sIL-6R elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 633863
• NCBI GeneID: 3570
• Molecular Weight: 40,237 Da
• NCBI Official Full Name: Soluble interleukin-6 receptor, partial
• NCBI Official Synonym Full Names: interleukin 6 receptor
• NCBI Official Symbol: IL6R
• NCBI Official Synonym Symbols: IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• NCBI Protein Information: interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha
• UniProt Protein Name: Interleukin-6 receptor subunit alpha
• UniProt Gene Name: IL6R
• UniProt Synonym Gene Names: IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
• UniProt Entry Name: IL6RA_HUMAN