Standard Curve (Sample)
Human NAD-dependent deacetylase sirtuin-1 ELISA Kit | SIRT1O elisa kit
Human NAD-dependent deacetylase sirtuin-1 ELISA Kit
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human
Synonyms
NAD-dependent deacetylase sirtuin-1; N/A; Human NAD-dependent deacetylase sirtuin-1 ELISA Kit; SIRT1O; hSIRT1; SIR2-like protein 1; hSIR2; SIRT1; SIR2L1; SIRT1O elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human SIRT1. No significant cross-reactivity or interference was observed.
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
78.0 pg/mL -5000 pg/mL.
Preparation and Storage
Store at 4 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SIRT1O elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human SIRT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SIRT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SIRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SIRT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SIRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,066 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform b
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2; SIR2L1; SIR2alpha
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1
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Product Notes
The Human SIRT1O sirt1 (Catalog #AAA310204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA310204 ELISA Kit recognizes Human SIRT1O. It is sometimes possible for the material contained within the vial of "NAD-dependent deacetylase sirtuin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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