Application Data
(The purity of SIRT7 was determined to be >90% by densitometry. Approx. MW 46kDa)
SIRT7 recombinant protein
SIRT7, Active
Gene Names
SIRT7; SIR2L7
Applications
SIRT Assay
Purity
>90%
Synonyms
SIRT7; N/A; SIRT7, Active; SIRT7 recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>90%
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Sequence Positions
Full Length
Sequence Length
1749
Applicable Applications for SIRT7 recombinant protein
SIRT Assay
Species
Human
Tag
HIS tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of SIRT7 was determined to be >90% by densitometry. Approx. MW 46kDa)
Application Data
(The purity of SIRT7 was determined to be >90% by densitometry. Approx. MW 46kDa)
Application Data
(The specific activity of SIRT7 was determined to be 160 RLU/min/ng as per activity assay protocol)
Application Data
(The specific activity of SIRT7 was determined to be 160 RLU/min/ng as per activity assay protocol)
Related Product Information for SIRT7 recombinant protein
Description: Full-length recombinant human SIRT7 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription (1). Depletion of SIRT7 stops cell proliferation and triggers apoptosis. SIRT7 deacetylates p53 and increases cellular resistance to cytotoxic and oxidative stress (2).
Scientific Background: SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription (1). Depletion of SIRT7 stops cell proliferation and triggers apoptosis. SIRT7 deacetylates p53 and increases cellular resistance to cytotoxic and oxidative stress (2).
Product Categories/Family for SIRT7 recombinant protein
References
1. Ford, E. et al: Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription. Genes Dev. 20: 1075-1080, 2006. 2. Vakhrusheva, O. et al Sirt7 increases stress resistance of cardiomyocytes and prevents apoptosis and inflammatory cardiomyopathy in mice. Circ. Res. 102: 703-710, 2008.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~46 kDa
NCBI Official Full Name
Homo sapiens sirtuin 7 (SIRT7), mRNA
NCBI Official Synonym Full Names
sirtuin 7
NCBI Official Symbol
SIRT7
NCBI Official Synonym Symbols
SIR2L7
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-7
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-7
UniProt Gene Name
SIRT7
UniProt Synonym Gene Names
SIR2L7
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Product Notes
The SIRT7 sirt7 (Catalog #AAA73143) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's SIRT7 can be used in a range of immunoassay formats including, but not limited to, SIRT Assay. Researchers should empirically determine the suitability of the SIRT7 sirt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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