Standard Curve (Sample)
Human CD150 ELISA Kit | SLAMF1 elisa kit
Human CD150 ELISA Kit
Gene Names
SLAMF1; SLAM; CD150; CDw150
Reactivity
Human
Applications
ELISA
Synonyms
CD150; N/A; Human CD150 ELISA Kit; SLAM; Signaling lymphocytic activation molecule; CDw150; IPO-3; SLAMF1 elisa kit
Reactivity
Human
Specificity
The Human CD150 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD150 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
335
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for SLAMF1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SLAMF1 elisa kit
Principle of the Assay||The Human CD150 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD150 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD150 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD150 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD150 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD150 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
Background/Introduction: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,292 Da
NCBI Official Full Name
Signaling lymphocytic activation molecule
NCBI Official Synonym Full Names
signaling lymphocytic activation molecule family member 1
NCBI Official Symbol
SLAMF1
NCBI Official Synonym Symbols
SLAM; CD150; CDw150
NCBI Protein Information
signaling lymphocytic activation molecule
UniProt Protein Name
Signaling lymphocytic activation molecule
UniProt Gene Name
SLAMF1
UniProt Synonym Gene Names
SLAM
UniProt Entry Name
SLAF1_HUMAN
Similar Products
Product Notes
The Human SLAMF1 slamf1 (Catalog #AAA261701) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261701 ELISA Kit recognizes Human SLAMF1. AAA Biotech's CD150 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the SLAMF1 slamf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD150, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.