Rat Secondary Lymphoid Tissue Chemokine ELISA Kit | SLC elisa kit

Rat Secondary Lymphoid Tissue Chemokine ELISA Kit

Cat. Num. AAA84944

• Gene Names: CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• Reactivity: Rat
• Synonyms: Secondary Lymphoid Tissue Chemokine; N/A; Rat Secondary Lymphoid Tissue Chemokine ELISA Kit; SLC elisa kit

• Reactivity: Rat
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml

• Intended Uses: This CCL-21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CCL-21. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for SLC elisa kit

Principle of the Assay: CCL-21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-21 antibody and an CCL-21-HRP conjugate. The assay sample and buffer are incubated together with CCL-21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-21 concentration since CCL-21 from samples and CCL-21-HRP conjugate compete for the anti-CCL-21 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-21 from the sample, fewer sites are left to bind CCL-21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-21 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SLC elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4128129
• NCBI GeneID: 6366
• UniProt Accession #: O00585
• Molecular Weight: 14,646 Da
• NCBI Official Full Name: secondary lymphoid tissue chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 21
• NCBI Official Symbol: CCL21
• NCBI Official Synonym Symbols: ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• NCBI Protein Information: C-C motif chemokine 21; beta chemokine exodus-2; secondary lymphoid tissue chemokine; Efficient Chemoattractant for Lymphocytes; small inducible cytokine subfamily A (Cys-Cys), member 21
• UniProt Protein Name: C-C motif chemokine 21
• UniProt Gene Name: CCL21
• UniProt Synonym Gene Names: SCYA21; UNQ784/PRO1600; SLC
• UniProt Entry Name: CCL21_HUMAN