Slit Homolog 3 (Slit3) Recombinant Protein | Slit3 recombinant protein

Recombinant Slit Homolog 3 (Slit3)

Cat. Num. AAA134229

• Gene Names: Slit3; Slil2; Slit1
• Reactivity: Mouse
• Applications: Western Blot, SDS-Page
• Purity: >98%
• Synonyms: Slit Homolog 3 (Slit3); N/A; Recombinant Slit Homolog 3 (Slit3); Slit3 recombinant protein

• Host: E. coli
• Reactivity: Mouse
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarkosyl, 5%Trehalose and Proclin300.
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for Slit3 recombinant protein: WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
• Source: Prokaryotic expression.
• Residues: Lys1348~Cys1517
• Tags: N-terminal His-Tag
• Subcellular Location: Secreted.
• Traits: Freeze-dried powder
• Original Concentration: 200µg/mL
• Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Predicted isoelectric point: 6.0
• Predicted Molecular Mass: 24.3kDa
• Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows: ; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. ; 2. Relative charge: The composition of amino acids may affects the charge of the protein. ; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. ; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. ; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 2-8 Degee C for one month.; Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 Degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-PAGE

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 226823283
• NCBI GeneID: 20564
• NCBI Accession #: NP_035542.2
• NCBI GenBank Nucleotide #: NM_011412.3
• UniProt Accession #: Q9WVB4; B1ATW4
• NCBI Official Full Name: slit homolog 3 protein
• NCBI Official Synonym Full Names: slit homolog 3 (Drosophila)
• NCBI Official Symbol: Slit3
• NCBI Official Synonym Symbols: Slil2; Slit1
• NCBI Protein Information: slit homolog 3 protein; slit-3
• UniProt Protein Name: Slit homolog 3 protein
• UniProt Gene Name: Slit3
• UniProt Synonym Gene Names: Slit-3; Slit3
• UniProt Entry Name: SLIT3_MOUSE