Standard Curve (Sample)
Rat Mothers Against Decapentaplegic Homolog 3 ELISA Kit | Smad3 elisa kit
Rat Mothers Against Decapentaplegic Homolog 3 ELISA Kit
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Rat
Synonyms
Mothers Against Decapentaplegic Homolog 3; N/A; Rat Mothers Against Decapentaplegic Homolog 3 ELISA Kit; Smad3/MADH3/SMAD family member 3/hMAD-3/hSMAD3/MAD homolog 3/mad protein homolog/MAD; mothers against decapentaplegic homolog 3/MAD; mothers against decapentaplegic homolog 3 (Drosophila)/mad3/Mad3/MADH3mad homolog JV15-2/mothers against decapentaplegic homolog 3/Mothers against DPP homolog 3; Smad3 elisa kit
Reactivity
Rat
Sequence Length
381
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Smad3 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Smad3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Smad3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Smad3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Smad3 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,722 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 3
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information
mothers against decapentaplegic homolog 3
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
UniProt Entry Name
SMAD3_HUMAN
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Product Notes
The Rat Smad3 smad3 (Catalog #AAA97747) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA97747 ELISA Kit recognizes Rat Smad3. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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