Canine Mothers Against Decapentaplegic Homolog 7 ELISA Kit | Smad7 elisa kit

Canine Mothers Against Decapentaplegic Homolog 7 ELISA Kit

Cat. Num. AAA93435

• Gene Names: SMAD7; CRCS3; MADH7; MADH8
• Reactivity: Canine
• Synonyms: Mothers Against Decapentaplegic Homolog 7; N/A; Canine Mothers Against Decapentaplegic Homolog 7 ELISA Kit; Smad7 elisa kit

• Reactivity: Canine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL

• Intended Uses: This Smad7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine Smad7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Smad7 elisa kit

Principle of the Assay: Smad7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Smad7 antibody and an Smad7-HRP conjugate. The assay sample and buffer are incubated together with Smad7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Smad7 concentration since Smad7 from samples and Smad7-HRP conjugate compete for the anti-Smad7 antibody binding site. Since the number of sites is limited, as more sites are occupied by Smad7 from the sample, fewer sites are left to bind Smad7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smad7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Smad7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 18418630
• NCBI GeneID: 4092
• Molecular Weight: 46,355 Da
• NCBI Official Full Name: Smad7
• NCBI Official Synonym Full Names: SMAD family member 7
• NCBI Official Symbol: SMAD7
• NCBI Official Synonym Symbols: CRCS3; MADH7; MADH8
• NCBI Protein Information: mothers against decapentaplegic homolog 7; hSMAD7; MAD homolog 8; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; MAD (mothers against decapentaplegic, Drosophila) homolog 7
• UniProt Protein Name: Mothers against decapentaplegic homolog 7
• UniProt Gene Name: SMAD7
• UniProt Synonym Gene Names: MADH7; MADH8; MAD homolog 7; Mothers against DPP homolog 7; MAD homolog 8; Mothers against DPP homolog 8; SMAD 7; Smad7; hSMAD7
• UniProt Entry Name: SMAD7_HUMAN