SMYD2 recombinant protein

Recombinant SMYD2 protein

Cat. Num. AAA59718

• Gene Names: SMYD2; KMT3C; HSKM-B; ZMYND14
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: SMYD2; N/A; Recombinant SMYD2 protein; KMT3C; HSKM-B; ZMYND14; SMYD2 recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Full length recombinant SMYD2 protein expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant SMYD2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 2ug Calf Thymus Octamer was incubated with different concentrations of recombinant SMYD2 protein in reaction buffer containing 50mM TrisCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 100uM 3H-SAM for 3 hours at room temperature. Activity was detected by Autoradiography.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

Application Data

(Recombinant SMYD2 protein activity assay 2 ug Calf Thymus Octamers was incubated with 0.5 ug and 1 ug SMYD2 protein respectivley in reaction buffer containing radioactive 3H-SAM for 3 hours at room temperature. Activity was detected by Autoradiography. *Our result showed that H4 may also be methylated by SMYD2.)

SDS-PAGE

(Recombinant SMYD2 protein gel SMYD2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for SMYD2 recombinant protein

Short Description: Recombinant SMYD2 protein (accession number NP_064582.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 50.7 kDa. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: SMYD2 (SET and MYND domain containing protein 2) is a lysine methyltransferase that methylates both histones and non-histone proteins. Specifically, SMYD2 methylates histone H3 Lys4 (H3K4me1) and dimethylates histone H3 Lys36 (H3K36me2). SMYD2 also exhibits methyltransferase activity toward non-histone proteins such as p53 and pRb. SMYD2 monomethylates Lys370 of p53, leading to decreased DNA-binding activity and subsequent inhibition of p53-mediated transcriptional regulation. SMYD2 also monomethylates Lys860 of pRb.

Product Categories/Family for SMYD2 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT)

NCBI and Uniprot Product Information

• NCBI GI #: 188035871
• NCBI GeneID: 56950
• NCBI Accession #: NP_064582.2
• NCBI GenBank Nucleotide #: NM_020197
• UniProt Accession #: Q9NRG4; Q4V765; Q5VSH9; Q96AI4; B2R9P9; I6L9H7
• Molecular Weight: 50
• NCBI Official Full Name: N-lysine methyltransferase SMYD2
• NCBI Official Synonym Full Names: SET and MYND domain containing 2
• NCBI Official Symbol: SMYD2
• NCBI Official Synonym Symbols: KMT3C; HSKM-B; ZMYND14
• NCBI Protein Information: N-lysine methyltransferase SMYD2
• UniProt Protein Name: N-lysine methyltransferase SMYD2
• UniProt Gene Name: SMYD2
• UniProt Synonym Gene Names: KMT3C
• UniProt Entry Name: SMYD2_HUMAN