Chicken Suppressors Of Cytokine Signaling 3 ELISA Kit | SOCS3 elisa kit

Chicken Suppressors Of Cytokine Signaling 3 ELISA Kit

Cat. Num. AAA92443

• Gene Names: SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• Reactivity: Chicken
• Synonyms: Suppressors Of Cytokine Signaling 3; N/A; Chicken Suppressors Of Cytokine Signaling 3 ELISA Kit; SOCS3 elisa kit

• Reactivity: Chicken
• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for SOCS3 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This SOCS3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SOCS3. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY SOCS3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SOCS3 antibody and an SOCS3-HRP conjugate. The assay sample and buffer are incubated together with SOCS3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOCS3 concentration since SOCS3 from samples and SOCS3-HRP conjugate compete for the anti-SOCS3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOCS3 from the sample, fewer sites are left to bind SOCS3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOCS3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SOCS3 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 45439352
• NCBI GeneID: 9021
• NCBI Accession #: NP_003946.3
• NCBI GenBank Nucleotide #: NM_003955.3
• UniProt Accession #: O14543; O14509; Q5U0H7; Q6FI39
• Molecular Weight: 24,770 Da
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: SOCS3
• NCBI Official Synonym Symbols: CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• NCBI Protein Information: suppressor of cytokine signaling 3; CIS-3; STAT-induced STAT inhibitor 3; cytokine-induced SH2 protein 3; cytokine-inducible SH2 protein 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• UniProt Gene Name: SOCS3
• UniProt Synonym Gene Names: CIS3; SSI3; SOCS-3; CIS-3; SSI-3
• UniProt Entry Name: SOCS3_HUMAN