SOD1 recombinant protein

SOD1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257989

• Gene Names: SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SOD1; N/A; SOD1 Protein, Human, Recombinant (His Tag); Human SOD1/Superoxide Dismutase Protein (His Tag); ALS Protein, Human; ALS1 Protein, Human; HEL-S-44 Protein, Human; homodimer Protein, Human; hSod1 Protein, Human; IPOA Protein, Human; SOD Protein, Human; superoxide dismutase 1, soluble; SOD1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0. 5% trehalose, 5% mannitol and 0.01% Tween80

• Source: Human
• Predicted N Terminal: Met
• Protein Construction: A DNA sequence encoding the human SOD1 (NP_000445.1)(Ala 2-Gln 154) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: It is receommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is mesured by UV-Vis.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommended to aliquot the protein into smaller quantities for optimal storage.

SDS-PAGE

Related Product Information for SOD1 recombinant protein

Background: SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.

References

Murakami K, et al. (2011) SOD1 (copper/zinc superoxide dismutase) deficiency drives amyloid beta protein oligomerization and memory loss in mouse model of Alzheimer disease. J Biol Chem. 286(52):44557-68.Thompson M, et al. (2012) Paradoxical roles of serine racemase and D-serine in the G93A mSOD1 mouse model of amyotrophic lateral sclerosis. J Neurochem. 120(4):598-610.Magrana© J, et al. (2012) Mitochondrial dynamics and bioenergetic dysfunction is associated with synaptic alterations in mutant SOD1 motor neurons. J Neurosci. 32(1):229-42.Gertz B, et al. (2012) Nuclear localization of human SOD1 and mutant SOD1-specific disruption of survival motor neuron protein complex in transgenic amyotrophic lateral sclerosis mice. J Neuropathol Exp Neurol. 71(2):162-77.

NCBI and Uniprot Product Information

• NCBI GI #: 4507149
• NCBI GeneID: 6647
• NCBI Accession #: NP_000445.1
• NCBI GenBank Nucleotide #: NM_000454.4
• UniProt Accession #: P00441; Q16669; Q16711; Q16838; Q16839; Q16840; Q6NR85; A6NHJ0; D3DSE4
• Molecular Weight: The recombinant human SOD1 consisting of 161 amino acids and has a calculated molecular mass of 16.8 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 1, soluble
• NCBI Official Symbol: SOD1
• NCBI Official Synonym Symbols: ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
• NCBI Protein Information: superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; Cu/Zn superoxide dismutase; SOD, soluble; epididymis secretory protein Li 44; indophenoloxidase A; superoxide dismutase, cystolic
• UniProt Protein Name: Superoxide dismutase [Cu-Zn]
• UniProt Gene Name: SOD1
• UniProt Synonym Gene Names: hSod1
• UniProt Entry Name: SODC_HUMAN