Application Data
(Jurkat cells were placed at 4 degree C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.)
Calcein AM Solution | AM solution
Calcein AM Solution (100 uM)
Synonyms
Calcein AM; N/A; Calcein AM Solution (100 uM); AM solution
Samples
Cell samples
Detection Instrument
Flow Cytometry, Fluorescence Microscope
Dye Type
Calcein
Channel Set
FITC
Filter Set
FITC
Detection Principle
Calcein AM Solution (100 uM) can be used to distinguish living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot be retained in the cell through the cell membrane, while Calcein can emit strong green fluorescence (Ex/Em = 494nm/517nm). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. Compared with other live cell staining probes, Calcein AM is less toxic and does not affect cell differentiation and proliferation.
Preparation and Storage
Store at -20 degree C, shading light
Estimated Expiration: 12 months
Shipping: Ice bag
Estimated Expiration: 12 months
Shipping: Ice bag
Application Data
(Jurkat cells were placed at 4 degree C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.)
Application Data
(Jurkat cells were placed at 4 degree C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.)
Application Data
(Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.)
Application Data
(Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.)
Product Categories/Family for AM solution
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Product Notes
The AM (Catalog #AAA178137) is a Solution and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Calcein AM, Solution" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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