SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SOS1 recombinant protein
SOS1, 564-1049aa, Human, His-tag, Baculovirus
Gene Names
SOS1; GF1; HGF; NS4; GGF1; GINGF; SOS-1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SOS1; N/A; SOS1, 564-1049aa, Human, His-tag, Baculovirus; Son of sevenless homolog 1; GF1; GGF1; GINGF; HGF; NS4; SOS1 recombinant protein
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 7.5) containing 30% glycerol, 0.1M NaCl, 1mM DTT, 0.2mM MgCl2.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
564-1049aa
Sequence
ADPEEQMRLP SADVYRFAEP DSEENIIFEE NMQPKAGIPI IKAGTVIKLI ERLTYHMYAD PNFVRTFLTT YRSFCKPQEL LSLIIERFEI PEPEPTEADR IAIENGDQPL SAELKRFRKE YIQPVQLRVL NVCRHWVEHH FYDFERDAYL LQRMEEFIGT VRGKAMKKWV ESITKIIQRK KIARDNGPGH NITFQSSPPT VEWHISRPGH IETFDLLTLH PIEIARQLTL LESDLYRAVQ PSELVGSVWT KEDKEINSPN LLKMIRHTTN LTLWFEKCIV ETENLEERVA VVSRIIEILQ VFQELNNFNG VLEVVSAMNS SPVYRLDHTF EQIPSRQKKI LEEAHELSED HYKKYLAKLR SINPPCVPFF GIYLTNILKT EEGNPEVLKR HGKELINFSK RRKVAEITGE IQQYQNQPYC LRVESDIKRF FENLNPMGNS MEKEFTDYLF NKSLEIEPRN PKPLPRFPKK YSYPLKSPGV RPSNPRPGTH HHHHH
Applicable Applications for SOS1 recombinant protein
SDS-PAGE
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at +2 degree C to +8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for SOS1 recombinant protein
SOS1, also known as son of sevenless homolog 1, is a Ras and Rac guanine nucleotide exchange factor. This protein is composed of several important domains. The CDC25 and REM domains provide the catalytic activity of SOS1 towards Ras and the histone fold DH/PH (Dbl homology and Pleckstrin homology) domains function, in tandem, to stimulate GTP/GDP exchange for Rac. Also, binding of GTP activates Ras proteins, and subsequent hydrolysis of the bound GTP to GDP and phosphate inactivates signaling by these proteins. GTP binding can be catalyzed by guanine nucleotide exchange factors for RAS, and GTP hydrolysis can be accelerated by GTPase-activating proteins (GAPs). Recombinant human SOS1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for SOS1 recombinant protein
References
Baruzzi A., et al, (2015) J. Immunol. 195:4900-4912. Lioubin MN., et al, (1994) Mol. Cell. Biol. 14:5682-5691.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58.0kDa (495aa)
NCBI Official Full Name
son of sevenless homolog 1
NCBI Official Synonym Full Names
SOS Ras/Rac guanine nucleotide exchange factor 1
NCBI Official Symbol
SOS1
NCBI Official Synonym Symbols
GF1; HGF; NS4; GGF1; GINGF; SOS-1
NCBI Protein Information
son of sevenless homolog 1
UniProt Protein Name
Son of sevenless homolog 1
UniProt Gene Name
SOS1
UniProt Synonym Gene Names
SOS-1
Similar Products
Product Notes
The SOS1 sos1 (Catalog #AAA48972) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 564-1049aa with tag His-tag. AAA Biotech's SOS1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SOS1 sos1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPEEQMRLP SADVYRFAEP DSEENIIFEE NMQPKAGIPI IKAGTVIKLI ERLTYHMYAD PNFVRTFLTT YRSFCKPQEL LSLIIERFEI PEPEPTEADR IAIENGDQPL SAELKRFRKE YIQPVQLRVL NVCRHWVEHH FYDFERDAYL LQRMEEFIGT VRGKAMKKWV ESITKIIQRK KIARDNGPGH NITFQSSPPT VEWHISRPGH IETFDLLTLH PIEIARQLTL LESDLYRAVQ PSELVGSVWT KEDKEINSPN LLKMIRHTTN LTLWFEKCIV ETENLEERVA VVSRIIEILQ VFQELNNFNG VLEVVSAMNS SPVYRLDHTF EQIPSRQKKI LEEAHELSED HYKKYLAKLR SINPPCVPFF GIYLTNILKT EEGNPEVLKR HGKELINFSK RRKVAEITGE IQQYQNQPYC LRVESDIKRF FENLNPMGNS MEKEFTDYLF NKSLEIEPRN PKPLPRFPKK YSYPLKSPGV RPSNPRPGTH HHHHH. It is sometimes possible for the material contained within the vial of "SOS1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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