General Staphylococcus Aureus Protein A ELISA Kit | SPA elisa kit

Staphylococcus Aureus Protein A ELISA Kit

Cat. Num. AAA247873

• Reactivity: General
• Synonyms: Staphylococcus Aureus Protein A; N/A; Staphylococcus Aureus Protein A ELISA Kit; SPA elisa kit

• Reactivity: General
• Specificity: Capture antibody and detection antibody respectively identify different epitopes of the antigen, which maximizes the specificity of the reaction. No cross-reaction exists between the homologous antigens.
• Assay Type: Quantitative Sandwich

• Intra-assay Precision: 45024
• Inter-assay Precision: 45148

Standard Curve (Sample)

Related Product Information for SPA elisa kit

Background: Staphylococcus Aureus Protein A (SPA or Protein A) is a 42kDa surface protein named MSCRAMM(Microbial Surface Components Recognizing Adhesive Matrix Molecules), it is originally found in the cell wall of the bacteria Staphylococcus aureus. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. Protein A can bind with strong affinity to human IgG1, human IgG2, mouse IgG2a and IgG2b, and with medium affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. Protein A can not bind to human IgG3,IgD or react with human IgM, IgA, IgE.. Protein A is widely used in biomedicine field because of its strong affinity to immunoglobulins. Protein A used to produce antibodies in the medicine field are mostly the ones binding to stationary-phase Chromatographic Resin. A small amount of protein A used in affinity chromatography may fall off the chromatographic column, and enter into elution tank. Leakage of protein A may contaminate the antibodies during the production process. Protein A has the ability to interact with multiple hosts, which indicate it might be a virulence factor in Staphylococcus aureus infection. To ensure the quality of antibodies purified by Protein A affinity chromatography, we have successfully developed Protein A ELISA kit. The kit is also applicable for the quantitative measurement of protein A in the samples with highly concentrated antibody such as purified antibody end products.

Principle of the Assay: This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of SPA in samples. The microtiter plate has been pre-coated with chicken anti-SPA antibody, standards or samples are then added to the microtiter plate wells and SPA if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a Horseradish Peroxidase(HRP)-conjugated detection rabbit anti-SPA antibody is added to the wells, under 37? incubation, to form a precoated antibody-SPA-detection antibody sandwich complex. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total SPA bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of SPA in samples is then determined by comparing the O.D. of the samples to the standard curve.