Application Data
(immobilized protein to support the adhesion of B16 cells.When 5×10<sup>4</sup> cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED<sub>50</sub> for this effect is 21.0-83.9 ng/mL.)
Osteopontin/SPP-1 Recombinant Protein | SPP1 recombinant protein
Recombinant Mouse Osteopontin/SPP-1 Protein
Purity
>95% as determined by HPLC
Synonyms
Osteopontin/SPP-1; N/A; Recombinant Mouse Osteopontin/SPP-1 Protein; Spp1, Eta-1, Op, Spp-1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1; SPP1 recombinant protein
Host
HEK293 cells
Purity/Purification
>95% as determined by HPLC
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH7.4.
Sequence
LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVN
Species
Mouse
Tag
C-His
Endotoxin
<1EU/ug
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of B16 cells.When 5×104 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour
Preparation and Storage
Store at -20 degree C. Store the lyophilized protein at -20 degree C to -80 degree C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
Application Data
(immobilized protein to support the adhesion of B16 cells.When 5×10<sup>4</sup> cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED<sub>50</sub> for this effect is 21.0-83.9 ng/mL.)
Application Data
(immobilized protein to support the adhesion of B16 cells.When 5×10<sup>4</sup> cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED<sub>50</sub> for this effect is 21.0-83.9 ng/mL.)
Application Data
(The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.)
Application Data
(The purity of Mouse Osteopontin/SPP1 is greater than 95% as determined by SEC-HPLC.)
SDS-PAGE
(Recombinant Mouse Osteopontin/SPP1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa.)
SDS-PAGE
(Recombinant Mouse Osteopontin/SPP1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-60 kDa.)
Related Product Information for SPP1 recombinant protein
Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
Product Categories/Family for SPP1 recombinant protein
NCBI and Uniprot Product Information
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Product Notes
The SPP1 spp1 (Catalog #AAA283390) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: LPVKVTDSGS SEEKLYSLHP DPIATWLVPD PSQKQNLLAP QNAVSSEEKD DFKQETLPSN SNESHDHMDD DDDDDDDDGD HAESEDSVDS DESDESHHSD ESDETVTAST QADTFTPIVP TVDVPNGRGD SLAYGLRSKS RSFQVSDEQY PDATDEDLTS HMKSGESKES LDVIPVAQLL SMPSDQDNNG KGSHESSQLD EPSLETHRLE HSKESQESAD QSDVIDSQAS SKASLEHQSH KFHSHKDKLV LDPKSKEDDR YLKFRISHEL ESSSSEVN. It is sometimes possible for the material contained within the vial of "Osteopontin/SPP-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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