Standard Curve (Sample)
Mouse Steroid 5 Alpha Reductase ELISA Kit | SRD5a elisa kit
Mouse Steroid 5 Alpha Reductase ELISA Kit
Gene Names
LOC101508101; sr5a
Reactivity
Mouse
Synonyms
Steroid 5 Alpha Reductase; N/A; Mouse Steroid 5 Alpha Reductase ELISA Kit; SRD5a elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of SD5alphaR. No significant cross-reactivity or interference between SD5alphaR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SD5alphaR and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SRD5a elisa kit
Intended Uses: This SD5alphaR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SD5alphaR. This ELISA kit for research use only!
Principle of the Assay: SD5alphaR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SD5alphaR antibody and an SD5alphaR-HRP conjugate. The assay sample and buffer are incubated together with SD5alphaR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SD5alphaR concentration since SD5alphaR from samples and SD5alphaR-HRP conjugate compete for the anti-SD5alphaR antibody binding site. Since the number of sites is limited, as more sites are occupied by SD5alphaR from the sample, fewer sites are left to bind SD5alphaR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SD5alphaR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SD5alphaR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SD5alphaR antibody and an SD5alphaR-HRP conjugate. The assay sample and buffer are incubated together with SD5alphaR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SD5alphaR concentration since SD5alphaR from samples and SD5alphaR-HRP conjugate compete for the anti-SD5alphaR antibody binding site. Since the number of sites is limited, as more sites are occupied by SD5alphaR from the sample, fewer sites are left to bind SD5alphaR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SD5alphaR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,989 Da
NCBI Official Full Name
steroid 5-alpha reductase
NCBI Official Symbol
LOC101508101
NCBI Official Synonym Symbols
sr5a
NCBI Protein Information
very-long-chain enoyl-CoA reductase-like; steroid 5-alpha reductase
UniProt Protein Name
Steroid 5-alpha reductase
UniProt Gene Name
sr5a
UniProt Entry Name
Q8GTD7_CICAR
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Product Notes
The Mouse SRD5a sr5a (Catalog #AAA86227) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA86227 ELISA Kit recognizes Mouse SRD5a. It is sometimes possible for the material contained within the vial of "Steroid 5 Alpha Reductase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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