General 5-hydroxytryptamine ELISA Kit | ST/5-HT elisa kit
5-hydroxytryptamine ELISA Kit
Reactivity
General
Synonyms
5-hydroxytryptamine; N/A; 5-hydroxytryptamine ELISA Kit; ST/5-HT (Serotonin/5-Hydroxytryptamine); ST/5-HT elisa kit
Reactivity
General
Specificity
Specifically recognize ST/5-HT, no obvious cross reaction with other analogues
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for ST/5-HT elisa kit
Background: Serotonin (5-hydroxytryptamine, 5-HT) is a central nervous system monoamine implicated in a number of physiological functions, including sleep, mood, appetite, anxiety, and neurovegetative control.Serotonin acts through the activation of G protein receptors called serotonin receptors (5-HTRs).Seven 5-HTR subclasses (5-HTR1-7) have been described to date.Serotonin receptor 4 (5-HTR4) couples to Galphas(3),with stimulation of the receptor leading to adenylyl cyclase activation, elevation of cyclic AMP levels and downstream PKA activation.The corresponding HTR4 gene exhibits a complex structure, which includes a large number of splice variants that seem to share the same pharmacological properties. 5-HTR4 is one of several receptors implicated in cognition, depression,and the development of Alzheimer's disease.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
