Guinea Pig Stromelysin-2 ELISA Kit | ST2 elisa kit

Guinea Pig Stromelysin-2 ELISA Kit

Cat. Num. AAA92499

• Gene Names: MMP10; SL-2; STMY2
• Reactivity: Guinea Pig
• Synonyms: Stromelysin-2; N/A; Guinea Pig Stromelysin-2 ELISA Kit; ST2 elisa kit

• Reactivity: Guinea Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP-10. No significant cross-reactivity or interference between MMP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-10 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for ST2 elisa kit

Principle of the Assay: MMP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP-10 antibody and an MMP-10-HRP conjugate. The assay sample and buffer are incubated together with MMP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-10 concentration since MMP-10 from samples and MMP-10-HRP conjugate compete for the anti-MMP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-10 from the sample, fewer sites are left to bind MMP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-10 concentration in each sample is interpolated from this standard curve.

Intended Uses: This MMP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig MMP-10. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 4505205
• NCBI GeneID: 4319
• NCBI Accession #: NP_002416.1
• NCBI GenBank Nucleotide #: NM_002425.2
• UniProt Accession #: P09238; Q53HH9; B2R9X9
• Molecular Weight: 54,151 Da
• NCBI Official Full Name: stromelysin-2 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 10 (stromelysin 2)
• NCBI Official Symbol: MMP10
• NCBI Official Synonym Symbols: SL-2; STMY2
• NCBI Protein Information: stromelysin-2; MMP-10; transin 2; transin-2; stromelysin 2; matrix metalloprotease 10; matrix metalloproteinase-10; matrix metalloproteinase 10 (stromelysin 2)
• UniProt Protein Name: Stromelysin-2
• UniProt Gene Name: MMP10
• UniProt Synonym Gene Names: STMY2; SL-2; MMP-10
• UniProt Entry Name: MMP10_HUMAN