Application Data
(Intracellular Staining of PBMC. 0.5 ug of antibody was used. Red: Human TLR3 PE. Green: Isotype control (mouse IgG1PE) was used. Intracellular FLOW kit was used.)
FLOW IntraCellular Staining Kit
FLOW IntraCellular Staining Kit (IC FLOW)
Applications
Flow Cytometry, Functional Assay
Synonyms
FLOW IntraCellular; N/A; FLOW IntraCellular Staining Kit (IC FLOW); FLOW IntraCellular staining kit
Kit Components
Fixation Buffer (1X) - 60 ml, Permeabilization Buffer (10X)- 2 X 60 ml, Staining Buffer - (1X) - 60 ml
Applicable Applications for FLOW IntraCellular staining kit
FCM/FACS (Flow Cytometry)
Preparation and Storage
Store at 2-8 degree C
Application Data
(Intracellular Staining of PBMC. 0.5 ug of antibody was used. Red: Human TLR3 PE. Green: Isotype control (mouse IgG1PE) was used. Intracellular FLOW kit was used.)
Application Data
(Intracellular Staining of PBMC. 0.5 ug of antibody was used. Red: Human TLR3 PE. Green: Isotype control (mouse IgG1PE) was used. Intracellular FLOW kit was used.)
Related Product Information for FLOW IntraCellular staining kit
The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First, live cells can be fixed with the Fixation buffer, which cross-links the protein. Second, the 1X Permeabilization Buffer creates holes in the membrane, allowing the antibodies enter into the cells effectively. Washing steps and antibody dilutions are done using 1X Permeabilization Buffer. The final cell resuspension prior to analysis is done using The Staining Buffer. Permeabilization Buffer is supplied as a 10X solution. Prior to use, this should be diluted 10 fold in distilled water. (1ml of Permeabilization Buffer with 9 ml of distilled water). 10X Permeabilization buffer has natural tendency to precipitate, however, its function is not affected. 1X solution can be filtered.
Product Categories/Family for FLOW IntraCellular staining kit
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Product Notes
The FLOW IntraCellular (Catalog #AAA78396) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FLOW IntraCellular can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the FLOW IntraCellular for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLOW IntraCellular, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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