ICC (Immunocytochemistry)
(Figure. Enhanced Antifade Mounting Medium with DAPI on paraffin sections of mouse lung tissue. Primary antibody CD34 (Cat #: dilution ratio 1:200), Dylight 594, Goat Anti-Rabbit IgG (Cat #: dilution ratio 1:200).)
Enhanced Antifade Mounting Medium with DAPI Staining Reagent | DAPI staining reagent
Enhanced Antifade Mounting Medium with DAPI
Applications
Immunohistochemistry
Synonyms
Enhanced Antifade Mounting Medium with DAPI; N/A; Antifade Mounting Medium; Anti-fluorescence quenching agent; Antifade Mounting Medium with dapi; dapi; Anti-fluorescence quenching agent with dapi; BMU; BMU107; DAPI staining reagent
Applicable Applications for DAPI staining reagent
IHC (Immunohistochemistry)
Features & Benefits
Strong anti-fluorescence quenching effect: Stored away from light for more than 2 weeks, the original luminous intensity can still be maintained.
High compatibility: The carefully optimized formula is compatible with a variety of fluorescent dyes.
Easy to operate: Ready-to-use, no configuration required.
High compatibility: The carefully optimized formula is compatible with a variety of fluorescent dyes.
Easy to operate: Ready-to-use, no configuration required.
Assay Procedure
I. Adherent cells:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the glass slide, coat the cover glass with the cells, and let the cells contact the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the cell samples under the fluorescence microscope.
II. Tissue slices:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the tissue slice, coat it with a cover glass, let the slice touch the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the tissue slices under the fluorescence microscope.
III Other samples:
For other samples, refer to cell samples or tissue slices for manipulation.
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the glass slide, coat the cover glass with the cells, and let the cells contact the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the cell samples under the fluorescence microscope.
II. Tissue slices:
1. After dyeing, suck up the liquid.
2. Add a drop of Enhanced Antifade Mounting Medium with DAPI on the tissue slice, coat it with a cover glass, let the slice touch the mounting fluid, and try to avoid air bubbles.
3. Then you can observe the tissue slices under the fluorescence microscope.
III Other samples:
For other samples, refer to cell samples or tissue slices for manipulation.
Preparation and Storage
Store at -20 degree C for 12 months, protected from light.
ICC (Immunocytochemistry)
(Figure. Enhanced Antifade Mounting Medium with DAPI on paraffin sections of mouse lung tissue. Primary antibody CD34 (Cat #: dilution ratio 1:200), Dylight 594, Goat Anti-Rabbit IgG (Cat #: dilution ratio 1:200).)
ICC (Immunocytochemistry)
(Figure. Enhanced Antifade Mounting Medium with DAPI on paraffin sections of mouse lung tissue. Primary antibody CD34 (Cat #: dilution ratio 1:200), Dylight 594, Goat Anti-Rabbit IgG (Cat #: dilution ratio 1:200).)
Application Data
Application Data
Related Product Information for DAPI staining reagent
Description: Enhanced Antifade Mounting Medium with DAPI is compatible with a wide range of fluorescent dyes and protects the entire visible and infrared spectrum from fluorescence signal quenching.
Background: The irreversible destruction of fluorescent molecules caused by environmental influences such as light, pH and temperature, or the interaction of fluorescent molecules with other molecules is fluorescence quench. Fluorescence quench results in the weakening of the visible signal when observed by fluorescence microscope and laser confocal microscope. Thus, SuperKine Enhanced Antifade Mounting Medium with DAPI is used to seal fluorescent tissue and cell samples with DAPI and unique anti-fluorescence quencher ingredients and compatible with a variety of fluorescent dyes to protect the entire visible and infrared spectrum and prevent fluorescence signal quenching. The original luminescence intensity can be maintained more than 2 weeks when the sample is stored at 4 degrees C or -20 degrees C.
Background: The irreversible destruction of fluorescent molecules caused by environmental influences such as light, pH and temperature, or the interaction of fluorescent molecules with other molecules is fluorescence quench. Fluorescence quench results in the weakening of the visible signal when observed by fluorescence microscope and laser confocal microscope. Thus, SuperKine Enhanced Antifade Mounting Medium with DAPI is used to seal fluorescent tissue and cell samples with DAPI and unique anti-fluorescence quencher ingredients and compatible with a variety of fluorescent dyes to protect the entire visible and infrared spectrum and prevent fluorescence signal quenching. The original luminescence intensity can be maintained more than 2 weeks when the sample is stored at 4 degrees C or -20 degrees C.
Product Categories/Family for DAPI staining reagent
Similar Products
Product Notes
The DAPI (Catalog #AAA332529) is a Staining Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Enhanced Antifade Mounting Medium with DAPI can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the DAPI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enhanced Antifade Mounting Medium with DAPI, Staining Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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