Application Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and Annexin V-FITC.)
Propidium Iodide (PI) Staining Reagent | PI staining reagent
Propidium Iodide (PI) Staining Solution
Synonyms
Propidium Iodide (PI); N/A; Propidium Iodide (PI) Staining Solution; PI staining reagent
Preparation and Storage
Store at 2-8°C for up to one year in dark.
Cautions:
1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
Cautions:
1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.
Application Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and Annexin V-FITC.)
Application Data
(Jurkat cells were treated with 1uM Camptothecin and detected by this reagent and Annexin V-FITC.)
Related Product Information for PI staining reagent
Background/Introduction: The Propidium Iodide (PI) Staining Solution is developed to identify apoptotic and necrotic cells. Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Propidium Iodide (PI) and Annexin V.
Product Categories/Family for PI staining reagent
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Product Notes
The PI (Catalog #AAA174679) is a Staining Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Propidium Iodide (PI), Staining Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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