STAT3 recombinant protein

STAT3 recombinant protein

Cat. Num. AAA14243

• Gene Names: STAT3; APRF; HIES
• Applications: Western Blot
• Purity: >95%
• Synonyms: STAT3; N/A; STAT3 recombinant protein; APRF; FLJ20882; MGC16063

• Host: Sf9 insect cells
• Purity/Purification: >95%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 4978

• Applicable Applications for STAT3 recombinant protein: Kinase Assay, Western Blot (WB)
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; ; Ships in dry ice.; ; Stability: 1 year at -70°C from date of shipment.

SDS-PAGE

(The purity was determined to be >95% by densitometry. Approximately MW 120kDa)

Related Product Information for STAT3 recombinant protein

Recombinant full-length human STAT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF (1). This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes (2).

Product Categories/Family for STAT3 recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins

References

1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760

NCBI and Uniprot Product Information

• NCBI GI #: 47080104
• NCBI GeneID: 6774
• NCBI Accession #: NP_644805.1
• NCBI GenBank Nucleotide #: NM_139276
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8
• Molecular Weight: ~120 kDa
• NCBI Official Full Name: Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES
• NCBI Protein Information: signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN