Application Data
(The purity of STAT5B protein was determined to be >90% by densitometry. Apporx. MW 95kDa.)
STAT5B recombinant protein
STAT5B Protein
Gene Names
STAT5B; STAT5
Applications
SDS-Page
Purity
>90%
Synonyms
STAT5B; N/A; STAT5B Protein; STAT5B recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>90%
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Sequence Length
5167
Applicable Applications for STAT5B recombinant protein
SDS-PAGE
Tag
N terminal HIS tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice.
Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of STAT5B protein was determined to be >90% by densitometry. Apporx. MW 95kDa.)
Application Data
(The purity of STAT5B protein was determined to be >90% by densitometry. Apporx. MW 95kDa.)
Related Product Information for STAT5B recombinant protein
Description: Recombinant full-length human STAT5B was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: STAT5B or signal transducer and activator of transcription 5B is a member of the STAT family of transcription factors. STATs are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B is activated in response to a variety of cytokines (1). The JAK/STAT pathway mediates a key signal from the somatic gonad that regulates male germline sexual development (2). STAT5B is critical for maintenance of tolerance in vivo and it is proposed that STAT5B is probably activated by IL2R.
Scientific Background: STAT5B or signal transducer and activator of transcription 5B is a member of the STAT family of transcription factors. STATs are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B is activated in response to a variety of cytokines (1). The JAK/STAT pathway mediates a key signal from the somatic gonad that regulates male germline sexual development (2). STAT5B is critical for maintenance of tolerance in vivo and it is proposed that STAT5B is probably activated by IL2R.
Product Categories/Family for STAT5B recombinant protein
References
1. Wang, D.et.al: Naturally occurring dominant negative variants of Stat5.Molec. Cell Biol. 16: 6141-6148, 1996. 2. Wawersik, M.et.al: Somatic control of germline sexual development is mediated by the JAK/STAT pathway. (Letter) Nature 436: 563-567, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Approximately 95 kDa
NCBI Official Full Name
Homo sapiens signal transducer and activator of transcription 5B, mRNA
NCBI Official Synonym Full Names
signal transducer and activator of transcription 5B
NCBI Official Symbol
STAT5B
NCBI Official Synonym Symbols
STAT5
NCBI Protein Information
signal transducer and activator of transcription 5B
UniProt Protein Name
Signal transducer and activator of transcription 5B
UniProt Gene Name
STAT5B
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Product Notes
The STAT5B stat5b (Catalog #AAA73148) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STAT5B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the STAT5B stat5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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