TMB 1-Component HRP Membrane Substrate | SUBM substrate
TMB 1-Component HRP Membrane Substrate
Synonyms
TMB 1-Component HRP Membrane Substrate; N/A; TMB 1-Component HRP Membrane Substrate (SUBM); SUBM substrate
Specificity
• Faint yellow to pink liquid
• 1X ready to use
• 1X ready to use
Instructions
1. Perform electro-blotting procedure.
2. Block membranes 4 hours-overnight.
3. Probe membranes with antibodies and HRP conjugate.
4. Wash membranes after each antibody incubation step. Always transfer to a clean container for substrate development step.
5. Bring SUBM to room temperature; protect it from light.
6. Add SUBM to cover the membrane surface.
7. Incubate SUBM at room temperature for 5-20 minutes.
8. Monitor the substrate color development to visualize the target peptide and protein bands.
9. Stop the color development reaction by transferring the membrane to diH2 O. Change the solution several times to ensure complete removal of all soluble TMB components.
10. Analyze the data.
2. Block membranes 4 hours-overnight.
3. Probe membranes with antibodies and HRP conjugate.
4. Wash membranes after each antibody incubation step. Always transfer to a clean container for substrate development step.
5. Bring SUBM to room temperature; protect it from light.
6. Add SUBM to cover the membrane surface.
7. Incubate SUBM at room temperature for 5-20 minutes.
8. Monitor the substrate color development to visualize the target peptide and protein bands.
9. Stop the color development reaction by transferring the membrane to diH2 O. Change the solution several times to ensure complete removal of all soluble TMB components.
10. Analyze the data.
Preparation and Storage
Store at 2-8 degree C. Protect from light.
Related Product Information for SUBM substrate
Description: TMB 1-Component HRP Membrane Substrate (SUBM) is suitable for membrane applications using horseradish peroxidase (HRP) as the conjugated detection enzyme. SUBM should not be used for microwell (ELISA) applications.
TMB 1-Component HRP Membrane Substrate is a one-component for-mulation containing stabilized 3,3’,5,5’-tetramethylbenzidine (TMB). The TMB substrate is oxidized by the peroxidase enzyme to yield an insoluble dark blue reaction product. SUBM is supplied ready-to-use at 1X.
Best results are obtained by equilibrating TMB 1-Component HRP Membrane Substrate to room temperature (25°C) prior to use. After probing with the antibody and HRP reagents, wash membrane thoroughly and transfer the membrane into a clean container. Cover the membrane surface with ample amount of SUBM and incubate 5-20 minutes. The substrate will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product.
For best results, monitor the substrate color development process until the target protein bands are visible. To stop the reaction, rinse the membrane with reagent quality water. If the reaction proceeds too long, there will be excessive background staining and diminished resolution of the target peptide or protein banding regions. If the color development is too rapid or intense, it is recommended to dilute the antibodies or conjugates or shorten the incubation period.
TMB 1-Component HRP Membrane Substrate is a one-component for-mulation containing stabilized 3,3’,5,5’-tetramethylbenzidine (TMB). The TMB substrate is oxidized by the peroxidase enzyme to yield an insoluble dark blue reaction product. SUBM is supplied ready-to-use at 1X.
Best results are obtained by equilibrating TMB 1-Component HRP Membrane Substrate to room temperature (25°C) prior to use. After probing with the antibody and HRP reagents, wash membrane thoroughly and transfer the membrane into a clean container. Cover the membrane surface with ample amount of SUBM and incubate 5-20 minutes. The substrate will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product.
For best results, monitor the substrate color development process until the target protein bands are visible. To stop the reaction, rinse the membrane with reagent quality water. If the reaction proceeds too long, there will be excessive background staining and diminished resolution of the target peptide or protein banding regions. If the color development is too rapid or intense, it is recommended to dilute the antibodies or conjugates or shorten the incubation period.
Product Categories/Family for SUBM substrate
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Product Notes
The SUBM (Catalog #AAA52745) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TMB 1-Component HRP Membrane Substrate, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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