Sheep Soluble Urokinase Plasminogen Activator Receptor ELISA Kit | suPAR elisa kit

Sheep Soluble Urokinase Plasminogen Activator Receptor ELISA Kit

Cat. Num. AAA247579

• Reactivity: Sheep
• Synonyms: Soluble Urokinase Plasminogen Activator Receptor; N/A; Sheep Soluble Urokinase Plasminogen Activator Receptor ELISA Kit; suPAR elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of SuPAR. No significant cross-reactivity or interference between SuPAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SuPAR and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 301
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for suPAR elisa kit

Principle of the Assay: SuPAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SuPAR antibody and an SuPAR-HRP conjugate. The assay sample and buffer are incubated together with SuPAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SuPAR concentration since SuPAR from samples and SuPAR-HRP conjugate compete for the anti-SuPAR antibody binding site. Since the number of sites is limited, as more sites are occupied by SuPAR from the sample, fewer sites are left to bind SuPAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SuPAR concentration in each sample is interpolated from this standard curve.

Intended Uses: This SuPAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep SuPAR. This ELISA kit for research ly, not for therapeutic or test applications!

Product Categories/Family for suPAR elisa kit

Signal Transduction; Cancer; Cell Biology; Cardiovascular; Stem Cells; Developmental Biology

NCBI and Uniprot Product Information

• NCBI GI #: 13641309
• Molecular Weight: 31,263 Da
• NCBI Official Full Name: soluble urokinase plasminogen activator receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR; U-PAR; uPAR