Human Synaptopodin ELISA Kit | SYNPO elisa kit
Human Synaptopodin ELISA Kit
Reactivity
Human
Synonyms
Synaptopodin; N/A; Human Synaptopodin ELISA Kit; SYNPO elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This SYNPO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SYNPO. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for SYNPO elisa kit
Principle of the Assay: SYNPO ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SYNPO. Standards or samples are then added to the microtiter plate wells and SYNPO if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SYNPO present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SYNPO are added to each well to "sandwich" the SYNPO immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SYNPO and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SYNPO concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SYNPO elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
73,667 Da
NCBI Official Full Name
synaptopodin
UniProt Protein Name
Synaptopodin
UniProt Gene Name
SYNPO
UniProt Synonym Gene Names
KIAA1029
UniProt Entry Name
SYNPO_HUMAN
