Amyloid Beta Peptide 1-42 (HFIP treated) Synthetic Protein | APP synthetic protein

Human Synthetic Amyloid Beta Peptide 1-42 (HFIP treated)

Cat. Num. AAA254100

• Gene Names: APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Applications: Western Blot
• Purity: >98%
• Synonyms: Amyloid Beta Peptide 1-42 (HFIP treated); N/A; Human Synthetic Amyloid Beta Peptide 1-42 (HFIP treated); Amyloid Beta Peptide 1-42 (HFIP treated) Monomers; Amyloid Beta; Abeta Protein; Abeta peptide; Amyloid beta peptide; Beta amyloid peptide; amyloid beta precursor protein peptide; APP; APP synthetic protein

• Purity/Purification: >98%
• Form/Format: Dry powder. See "Other Resources" for re-suspension instructions/protocol.
• Sequence Positions: 42 amino acids
• Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

• Applicable Applications for APP synthetic protein: Invitro Assay, InVitro Assay, WB (Western Blot)
• Species: Human
• Conjugation: No Tag
• Research Area: Neuroscience, Neurodegeneration, Alzheimer's Disease, Amyloid
• Cellular Localization: Cell Membrane, Intracellular Vesicles
• Certificate of Analysis: Certified >98% pure using mass spec and HPLC.
• Preparation and Storage: Store at -80 degree C

Application Data

(AFMofamyloidbeta1-42monomers(AAA254100,left),oligomers andfibrils .Atomicforcemicroscopyanalysisof1.0mg/mLsamplesdilutedto0.1mg/mLindH2O,mountedonfreshlycleavedmica,washed,driedandanalyzedwithtappingmode.Representativeimagesare2.5x2.5umx-ywithaz-rangeof10nm.)

Application Data

(TEMofamyloidbeta1-42monomers(AAA254100,left),oligomers andfibrils .Negativestaintransmissionelectronmicroscopyimagesacquiredat80Kvoncarboncoated400meshcoppergridsusingphosphotungsticacidanduranylacetatestain.Scalebar=100nm.)

Application Data

(Amyloidbeta1-42oligomers andfibrils showadose-dependenttoxicitytoprimaryratcorticalneurons,butnotmonomers(AAA254100).Survivalofratprimarycorticalneurons14daysaftertreatmentwithdifferentconcentrationsof(A)monomers,(B)oligomersor(C)fibrilsquantifiedbyMAP2positiveneuronsandexpressedasapercentageofcontrol.FibrilsandrespectivevehiclecontrolswereinitiallysonicatedinaBioruptor.Testconditionswereruninthesameplateasuntreatedcontrolandvehiclecontrols,whichconsistedofbufferwithoutamyloidbeta1-42protein.Dataexpressedasmean+/-s.e.m.(n=6).Aglobalanalysisofthedatawasperformedusingaone-wayANOVAfollowedbyDunnett’stest;**p)

WB (Western Blot)

(Western Blotofamyloidbeta1-42monomers(AAA254100,left),oligomers andfibrils usinganti-amyloidbeta6E10antibody.Amyloidbetaconstructsat160pmolwererunon4-12%Bis-TrisSDS-PAGE,transferredtonitrocelluloseinthepresenceof0.02%v/vTween-20,andblottedwith1:1000mouse6E10primaryantibody(Biolegend).OligomersobservedunderTEM/AFMshowdistinctdimer/trimerbandsaswellasasignalfrom~37-75kDa(middle).FibrilsobservedunderTEM/AFMshowasignalgreaterthan100kDaandadistinctsignalinthestackinggel(right).)

Application Data

(TEMofamyloidbeta1-42monomers(AAA254100).Negativestaintransmissionelectronmicroscopyimagesacquiredat80Kvoncarboncoated400meshcoppergridsusingphosphotungsticacidanduranylacetatestain.Scalebar=100nm.)

Related Product Information for APP synthetic protein

Our amyloid beta peptide 1-42 (Abeta42) is produced synthetically and treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) prior to drying which breaks down pre-formed fibrils and monomerizes the peptide, as previously published (1,2). Upon resuspension in DMSO/dH2O, our Abeta42 presents as a monomeric peptide without fibrils when observed under TEM, AFM and on a Western Blot with an anti-amyloid beta antibody. In contrast to AB42 oligomer and fibril constructs, our Abeta42 monomers were not toxic to primary rat cortical neurons.In the brain, amyloid beta peptide (Abeta) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of Abeta plaques in the brain is considered a hallmark of Alzheimer's disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (3). Soluble Abeta oligomers isolated from the brains of AD patients or those generated in vitro potently impaired synapse structure and function (4). Abeta oligomers generated in vitro were toxic to PC12 cells (2) and SH-SY5Y cells (5). Abeta was demonstrated to interact with tauopathies to affect neurodegeneration in AD patients (6) and accumulations of Abeta were shown to be associated with lower survival rates in Parkinson's disease patients with dementia (7).

Product Categories/Family for APP synthetic protein

Proteins

References

1. Stine et al. 2003. JBC. 278(13):11612-22. doi: 10.1074/jbc.M2102072002. Chromy et al. 2003. Biochemistry. 42:12749-12760. doi: 10.1021/bi030029q3. Panza et al. 2019. Nat Rev Neurol. 15:73-88 https://doi.org/10.1038/s41582-018-0116-64. Shankar et al. 2008. Nat Med. 14(8):837-842. doi: 10.1038/nm17825. Kayed et al. 2003. Science. 300(5618): 486-489. doi: 10.1126/science.10794696. Want et al. 2016. JAMA Neurol. 73(9):1070-7. doi: 10.1001/jamaneurol.2016.20787. Kotzbauer et al. 2012. Arch Neurol. 69(10): 1326-1331. doi: 10.1001/archneurol.2012.1608

NCBI and Uniprot Product Information

• NCBI GI #: 4502167
• NCBI GeneID: 351
• NCBI Accession #: NP_000475.1
• NCBI GenBank Nucleotide #: NM_000484.3
• UniProt Accession #: P05067; P09000; P78438; Q13764; Q13778; Q13793; Q16011; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3
• Molecular Weight: 86,943 Da
• NCBI Official Full Name: amyloid beta A4 protein isoform a
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein
• NCBI Official Symbol: APP
• NCBI Official Synonym Symbols: AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• NCBI Protein Information: amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide
• UniProt Protein Name: Amyloid beta A4 protein
• UniProt Gene Name: APP
• UniProt Synonym Gene Names: A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
• UniProt Entry Name: A4_HUMAN