Goat Thrombin Activatable Fibrinolysis Inhibitor ELISA Kit | TAFI elisa kit

Goat Thrombin Activatable Fibrinolysis Inhibitor ELISA Kit

Cat. Num. AAA89072

• Gene Names: Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• Reactivity: Goat
• Synonyms: Thrombin Activatable Fibrinolysis Inhibitor; N/A; Goat Thrombin Activatable Fibrinolysis Inhibitor ELISA Kit; TAFI elisa kit

• Reactivity: Goat
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Intended Uses: This TAFI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TAFI. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for TAFI elisa kit

Principle of the Assay: TAFI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TAFI antibody and an TAFI-HRP conjugate. The assay sample and buffer are incubated together with TAFI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAFI concentration since TAFI from samples and TAFI-HRP conjugate compete for the anti-TAFI antibody binding site. Since the number of sites is limited, as more sites are occupied by TAFI from the sample, fewer sites are left to bind TAFI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAFI concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7416967
• NCBI GeneID: 56373
• UniProt Accession #: Q9JHH6; Q5EBI3; Q9QZF0
• Molecular Weight: 48,871 Da
• NCBI Official Full Name: thrombin-activatable fibrinolysis inhibitor
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: Cpb2
• NCBI Official Synonym Symbols: CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• NCBI Protein Information: carboxypeptidase B2; carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
• UniProt Protein Name: Carboxypeptidase B2
• UniProt Gene Name: Cpb2
• UniProt Synonym Gene Names: Tafi; CPR; CPU; TAFI
• UniProt Entry Name: CBPB2_MOUSE